响应面法优化黄芪总多糖超声提取工艺

Optimization of Ultrasonic Extraction Technology of Astragalus Polysaccharide by Response Surface Methodology

目的：以黄芪总多糖提取率为指标,通过响应面设计法确定黄芪总多糖最佳超声提取工艺。方法：通过单因素和响应面设计相结合的方法,以料液比、超声温度、功率、提取时间为考察因素,优化黄芪总多糖提取工艺。结果：黄芪总多糖最佳超声提取工艺条件为：液料比12∶1,超声温度65℃,功率300 W,提取时间13 min,此条件下总多糖的平均得率为22.35%,理论预测的总多糖得率为22.46%,两者相差较小。因此,响应面优化所得的黄芪总多糖提取工艺具有可行性。结论：超声法提取黄芪总多糖稳定可靠,节能省时,可用于工业化大生产。

Objective：To approach an optimal ultrasonic extraction process of with the extraction rate of Astragalus polysaccharide as an index,by the response surface design method.Methods：In order to optimize the extraction process of Astragalus polysaccharide,by the combination of single factor and response surface design,the ratio of material to liquid,ultrasonic temperature,power and extraction time were studied.Results：The optimum ultrasonic extraction conditions of Astragalus polysaccharides were as follows：the ratio of liquid to material was 12∶1,ultrasonic temperature was 65℃ and power was 300 W for 13 min.Under these conditionsthe average yield of total polysaccharides was 22.35%,which was close to the predicted value 22.46%.The results showed that the extraction process of Astragalus polysaccharides was feasible.Conclusion：Ultrasonic extraction of Astragalus polysaccharides is simple in process,saving energy and time,which provides a theoretical basis for industrial production.