高效液相色谱测定生鲜乳甲砜霉素残留量的方法研究及应用

Determination of Residues of Thiamphenicol in Raw Milk by HPLC and Its Application

本文采用高效液相色法谱测定生鲜乳中甲砜霉素残留检测方法,结果表明:在20~500 ng/mL浓度范围内,相关系数r为0.999 9,色谱峰面积与药物的质量浓度呈良好的线性关系,加标回收率在75.1%~91.5%,RSD在4.7%~8.9%。此方法检测限为10μg/kg,定量限为10μg/kg,对宁夏75批生鲜乳排查,结果全部未检出。

HPLC was established to determine residues of thiamphenicol in raw milk. Within the mass concentration range of20-500 ng/mL, the good linearity was obtained（r=0.999 9）, the recovery was 75.1%-91.5% and the RSD was 4.7%-8.9%. The LOD was 10 μg/kg and LOQ was 10 μg/kg. Thiamphenicol was not detected in 75 batches of raw milk in Ningxia.