手足口病患儿外周血Ghrelin水平与TNF-α、IL-1β、IL-6的相关性研究

Relevance of TNF-α,IL-1β,IL-6 and Ghrelin in Children with Hand,Foot and Mouth Disease

目的:探讨手足口病患儿体内Ghrelin水平与TNF-α、IL-1β、IL-6水平的相关性。方法:选择2015-2016年于我院儿科就诊的手足口病患儿(研究组)和在体检中心进行体检的健康儿童(对照组)各100例,研究组患儿根据病情分为轻症组(无脑炎)68例和重症组(有脑炎)32例。采用酶联免疫吸附试验(ELISA)法测定三组儿童外周血中Ghrelin的含量,同时采用化学发光分析仪测定外周血中免疫细胞因子TNF-α、IL-1β、IL-6水平,分析手足口病患儿Ghrelin水平与TNF-α、IL-1β、IL-6水平的相关性。结果:Ghrelin水平重症组<轻症组<对照组(P均<0.05);IL-6、IL-1β、TNF-α水平重症组>轻症组>对照组(P均<0.05);手足口病患儿外周血Ghrelin水平与TNF-α、IL-1β、IL-6均呈负相关(r分别为-0.31、-0.33、-0.29,P均<0.05)。结论:Ghrelin可能对手足口病的炎症免疫反应起保护作用,Ghrelin可以作为预测手足口病预后的指标之一。

Objective： To investigate the relevance of TNF-α,IL-1β,IL-6 and Ghrelin Levels in children with hand,foot and mouth disease. Methods： Children with hand,foot and mouth disease（ study group） and the healthy children from the health center（ control group） with 100 cases in each from 2015 to 2016 of our hospital were chosen. According to the severity of illness,study group were divided into mild group（ without encephalitis） and severe group（ with encephalitis）. ELISA method was used to determine the content of three groups of children in the peripheral blood of Ghrelin,while chemiluminescence analyzer was used to determine TNF-α,IL-1β,IL-6 levels in peripheral blood of immune cells,and the correlation of Ghrelin and TNF-α,IL-1β,IL-6 levels were analyzed. Results：The Ghrelin level of severe group was lower than that of mild group,and the mild group was lower than that of control group,while IL-6,IL-1β,TNF-α levels of severe group were higher than mild group,and the mild group were higher than those of control group（ P〈0. 05）.The levels of Ghrelin in peripheral blood of children with hand,foot and mouth disease were negatively correlated with TNF-α,IL-1βand IL-6（ r =-0. 31,-0. 33,-0. 29,respectively,P〈0. 05）. Conclusion： Ghrelin may protect immune response inflammation of hand,foot and mouth disease. It can become a predicting prognosis factor.