胰腺导管单克隆上皮样干细胞的蛋白表达特征

Protein Expression of Monoclonal Epithelia Stem Cells Derived from Pancreatic Duct

该文研究1例源于成年大鼠的胰腺导管单克隆上皮样干细胞系的蛋白表达特征、染色体核型及致瘤性。RPMI-1640有血清扩增培养源于成年大鼠的胰腺导管单克隆上皮样干细胞至单层,分别采用流式细胞术或免疫荧光反应检测干细胞蛋白水平的表达特征。常规法制备染色体标本,采用Adobe Photoshop CS6软件进行核型分析。将干细胞移植在裸鼠体内,观察其致瘤性。结果显示,该源于成年大鼠的胰腺导管单克隆上皮样干细胞在蛋白水平表达CK19、Neuro D2、Oct4、PCNA及Nanog,不表达Pdx1、Pax4、Pax6、Maf A、Ptf1a、Ngn3、nestin、Sox2、CD34、CD45、insulin、glucagon、ghrelin、somatostatin及secretin。细胞是正常的二倍体细胞(2n=42),无致瘤性。这表明,该源于成年大鼠的胰腺导管单克隆上皮样干细胞系共表达CK19、Neuro D2、Oct4、PCNA及Nanog蛋白。

In this study, expression characteristics, karyotype and tumorigenicity of a monoclonal epithelia stem cell line derived from pancreatic duct of an adult rat were researched. Monoclonal epithelia stem cells derived from pancreatic duct of an adult rat were proliferated to a layer in serum supplemented RPMI-1640 medium. Expression characteristics of stem cells were determined using flow cytometry and immunofluorescence reaction. Chromosome sets of stem cells were examed using conventional method, and karyotype was analysed with Adobe Photoshop CS6 software. Tumorigenicity was observed by transplanting stem cells into nude mice. The results showed that monoclonal epithelia stem cells derived from pancreatic duct of a adult rat were positive for the CK19, Neuro D2, Oct4, PCNA and Nanog, and were negative for the Pdx1, Pax4, Pax6, Maf A, Ptf1 a, Ngn3, nestin, Sox2, CD34, CD45, insulin, glucagon, ghrelin, somatostatin and secretin protein expression. Stem cells were normal diploid（2 n=42）. And these stem cells had no oncogenicity. Here a monoclonal epithelia stem cell line derived from pancreatic duct of an adult rat that co-expressed the CK19, Neuro D2, Oct4, PCNA and Nanog protein was determined.