摘要
目的探讨姜黄素对顺铂诱导人肾小管上皮细胞系HK-2凋亡的影响。方法用MTT法观察HK-2增殖;用Western blot检测HK-2的凋亡蛋白。结果 1)顺铂呈剂量依赖性诱导HK-2凋亡(P<0.05)。2)在姜黄素和顺铂联合作用下,凋亡的HK-2明显减少,细胞存活率提高(P<0.05);HK-2中Bax蛋白表达降低(P<0.05);Bcl-2蛋白表达增加(P<0.05)。结论姜黄素可以通过调控Bax和Bcl-2蛋白的表达抑制顺铂引起的细胞凋亡。
Objective To investigate whether curcumin could inhibit cisplatin-induced apoptosis of HK-2 cells.Methods Using MTT methods to observe inhibitory effects of curcumin and cisplatin on the proliferation of HK-2 cell. Detecting caspases expression after curcumin and cisplatin treatment by Western blot. Results Cisplatin induced cell apoptosis,and the effect was dose-dependent（ P〈0. 05）. Under the synergistic effect of curcumin and cisplatin on HK-2 cell,the number of apoptosis decreased（ P〈0. 05）. In the same time the expression of Bcl-2 protein increased（ P〈0. 05） and the expression of Bcl-2 protein decreased（ P〈0. 05）. Conclusions Cisplatin enhances the apoptosis of HK-2 cell in a dose-dependent manner,and is inhibited by curcumin. Curcumin can antagonize cisplatin-induced apoptosis in HK-2 by regulating the expressions of Bax and Bcl-2 protein.
出处
《基础医学与临床》
CSCD
2018年第2期185-188,共4页
Basic and Clinical Medicine
基金
国家级大学生创新创业科研项目(20151036800)
