番泻叶造模对小鼠肠道微生物及酶活性的影响

Effects of Senna on the intestinal microbiota and enzyme activity in mice with spleen-deficiency

目的探讨灌胃番泻叶脾虚腹泻造模方法对小鼠肠道微生物及酶活性的影响。方法采用灌胃番泻叶水煎液进行小鼠脾虚造模。12只小鼠随机分为模型组和正常组,模型组灌胃番泻叶水煎液,正常组灌胃等量无菌水,期间观察小鼠的一般情况变化。灌胃7d后称重、无菌采集肠道内容物,分析肠道菌群及酶活性。结果与正常组相比,模型组小鼠肠道内容物的淀粉酶活性升高极显著(t=-53.0671,P=0.0000),纤维素酶活性和木聚糖酶活性下降显著(t=23.1230,P=0.0000;t=12.3900,P=0.0410),而蛋白酶活性变化不明显(t=1.3120,P=0.0521);模型组小鼠细菌总数显著减少(t=73.1601,P=0.0000),而大肠埃希菌、乳杆菌、双歧杆菌和真菌总数变化均不明显(t=2.3580,P=0.1432;t=2.2089,P=0.1127;t=2.2813,P=0.1037;t=9.3210,P=0.0510)。结论脾虚造模会使细菌总数减少,纤维素酶和木聚糖酶活性下降,淀粉酶活性升高。

Objective To explore the effects of Senna on gut microbes and enzyme activity in mice with spleen-deficiency.Methods Water decoction of Senna by gavage was adopted to make mouse models of spleen deficiency.Twelve experimental mice were randomly divided into the model group or control group,with 6 mice in each group.The model group was gavaged with water decoction of senna for 7 days,while the control group was given sterile water by gavage.The general changes of mice during the gavage were observed.After seven days,the mice were weighted and the intestinal contents were collected for analysis of intestinal flora and enzyme activity.Results Compared with the controls,the activity of amylase in model group significantly increased（P〈0.01）while of cellulase and xylanase decreased significantly（P〈0.01 or P〈0.05）,but that of protease did not show obvious change（P〈0.05）.The total number of bacteria in model group was significantly less than in control group（P〈0.01）,while those of Escherichia coli,Lactobacillus,Bifidobacteriumand fungi did not change obviously（P〈0.05）.Conclusion Spleen deficiency modeling can reduce the total number of bacteria and the activity of cellulase and xylanase,while increase the activity of amylase in mice.