基于CAD与3D打印技术设计制作的缓释PDGF-BB/HA-PLA/纤维蛋白胶三维复合支架在骨缺损治疗领域的实验研究

Experiment Research of Basing on CAD and 3D Printing Technology Design and Construct the Slow-release PDGF-BB/HA-PLA/fibrin Glue Three-dimensional Composite Scaffold for Bone Defect Treatment

目的:探讨利用CAD与3D打印技术设计制作的缓释PDGF-BB/HA-PLA/纤维蛋白胶三维复合支架作为工程骨,在骨缺损治疗领域的研究意义。方法:以新西兰大白兔的桡骨缺损为模板,利用Mimics18.0、Unigraphics NX10.0等CAD软件,建立所需支架的数字模型,然后以PLA(S2支架)和PGA为原料,借助3D打印技术制作HA-PLA支架,最后将PDGF-BB细胞悬液、纤维胶原蛋白胶将其填充、浸润制作出具有生物活性三维复合支架(S1支架)。同时对S1支架组(n=4)与S2支架组(n=4)进行验证分析。结果:术后12周处死兔后通过大体观察,S1支架组4例桡骨缺损均顺利愈合,S2支架组1例顺利愈合,2例延迟愈合,1例未愈合;术后第4、8、12周S1支架组的Lane-Sandhu X线评分、骨密度测量值均优于S2支架组,且差异具有显著性(P<0.05)。结论:利用CAD与3D打印技术设计制作的缓释PDGF-BB/HA-PLA/纤维蛋白胶三维复合支架,具有生物相容性好、安全性高、促进骨缺损愈合等优点,其在未来骨缺损治疗的领域发展前景广阔。

Objective： To explore the research significance of the basing on CAD and 3D printing technology design and construct the slow-release PDGF-BB/HA-PLA/fibrin glue three-dimensional composite scaffold as engineered bone in field of bone defect treatment. Methods： The New Zealand white rabbit were made as the template, though using Minfics18.0, Unigraphics NX10.0, and so on CAD softwares, the digital scaffold model was built, and then PLA （S2 scaffold） and PGA were made into HA-PLA by 3D printing technology, at last PDGF-BB cell suspension and fibrin glue were filled into bioactive three-dimensional composite scaffolds （S1 scaffold）. At the same time, S1 scaffold group （u=4） and S2 scaffold group （n=4） were verified and analysised. Results： Being put to death the rabbits by gross observation after 12 weeks, 6 cases of S1 scaffold group all healed smoothly, 1 case of S2 scaffold group healed smoothly, 2 cases of delayed healing, 1 case was nonunion; after 4, 8, 12 weeks S1 scaffold group Lane-Sandhu X-ray score, BMD measurements were superior to S2 scaffold group, and it was statistical difference （P〈0.05）. Conclusion： The basing on CAD and 3D printing technology design and construct the slow-release PDGF-BB/HA-PLA/fibrin glue three-dimensional composite scaffold as engineered bone in field of bone defect treatment, which have these advantages of good biocompatibility, high security and promote healing of bone defect, this kind of scaffold hold great promise for the treatment of bone defect in the future.