电针对大鼠在体海马长时程增强急性效应的影响

Acute Effect of Electroacupuncture on Hippocampal Long-term Potentiation of Rats in Vivo

目的:探讨单次电针刺激百会、神庭穴对大鼠海马内嗅区皮层穿通纤维到海马齿状回颗粒细胞层(PP-DG)通路场电位群峰电位(PS)波幅及长时程增强(LTP)的影响。方法:20只Wistar雄性大鼠按照体质量随机分为电针组和对照组,每组各10只。2组大鼠使用乌拉坦麻醉后,固定于脑立体定位仪上,记录PPDG通路场电位,期间使用配对脉冲刺激记录并使用θ节律刺激诱导LTP出现。之后电针组大鼠给予电针刺激百会、神庭穴,采用连续波、频率为2 Hz、强度为2 m A、时间为30 min,对照组不予干预。干预结束后立即将电针组大鼠按前述方法再次检测PP-DG通路场电位PS波幅及LTP。结果:与对照组比较,电针组PPDG通路场电位PS波幅及LTP均升高,差异具有统计学意义(P<0.05)。结论:电针刺激百会、神庭穴能够增高PP-DG通路场电位PS波幅,增强LTP诱导能力,该机制可能与电针刺激百会、神庭穴调节海马神经元的功能有关。

Objective：To investigate the effects of single electroacupuncture（EA） at Shenting and Baihui on hippocampal population spike（PS） response and long-term potentiation（LTP） of perforant path-dentate gyrus（PP-DG） granule cells synapse in anesthetized normal rat and explore the underlying mechanisms. Methods：A total of 20 male Wistar rats were randomly divided into the EA group（n=10） and the control group（n=10）. Rats were anesthetized with Urethane, and placed in a stereotaxic apparatus. The PS amplitude in response to perforant path stimulation was record. The paired pulse stimulation was given at two inter-pulse intervals, then theta burst stimulation was applied to perforant path before and after EA. Acupuncture needles were gently inserted into Shenting and Baihui of rats after acquiring steady response in dentate gyrus. Stimulation was applied to these acupoints with 2 m V of continuous wave（2 Hz） for 30 minutes using an EA apparatus. Results：Compared with the control group, the hippocampal PS amplitude and LTP were significant increased in EA group（P0.05）. Conclusion：EA at Shenting and Baihui could raise up the PS amplitude in response to perforant path stimulation, and enhance LTP, which may related to the modulating functions of interneuron in hippocampus.