器官移植生物样本库的建立及意义

Establishment and importance of the biological bank for samples from organ transplantation

目的探讨规范化的器官移植生物样本库建立对器官移植领域研究提供优质样本的应用价值。方法选取2014年9月-2016年9月天津市第一中心医院生物样本库经过采集、处理及保存流程储存的生物样本。样本均为依据国际环境与生物样本库协会(ISBER)和中国医药生物技术协会组织生物样本库分会制定的规范化生物样本。随机抽取200例恶性肿瘤肝移植患者保存的肿瘤样本石蜡切片,苏木素-伊红(HE)染色法检测肿瘤坏死程度,琼脂糖凝胶电泳条带分析肿瘤组织内DNA和RNA的完整性。结果随机抽检200例储存的恶性肿瘤样本中,HE染色切片病理分析显示其中192例(占96%)肿瘤面积≥80%。组织中RNA的A260/A280均在1.9~2.0,琼脂糖凝胶电泳显示28S及18S色带清晰,无拖尾现象,表明RNA的完整性良好。组织中DNA的A260/A280均在1.8~1.9,凝胶电泳检测显示DNA条带清晰无明显降解。结论建立规范化器官移植样本采集、处理及保存方法可为器官移植领域的科研提供优质的样本资源。

Objective To clear the applicable value of standardized protocols of sampling in organ transplantation which were established to obtain high quality samples. Methods From September 2014 to September 2016, samples in the Tianjin First Center Hospital biobank were studied, which were obtained and stored following the protocols of International Association of Environmental and Biological Samples（ISBER） and China Biosafety Society. Paraffin tumor sections from 200 patients who underwent liver transplant were studied. Tumor necrosis was assessed by HE staining. DNAs/RNAs were extracted from tumor tissues, which were quantified by gel electrophoresis. Results A total of 200 samples were included in this study. Tumors Area ≥ 80% was found in 192（96%） samples. The A260/A280 of RNAs in tissues was 1.9-2.0. Gel electrophoreses showed that the 28 S and 18 S bands were clear and no tailing, indicating that the RNA integrity was good. The A260/A280 of DNA in tissues was 1.8-1.9, and electrophoreses of the DNA showed clear and no obvious degradation. Conclusion Standardized collection, management and preservation for samples from organ transplantation may contribute to establish high quality sample resources for studies of this field.