摘要
目的采用响应面分析法(RSM)优化金毛狗脊总黄酮的提取工艺条件。方法采用Box-Benhnken中心组合设计,利用RSM法,分别研究乙醇浓度、药液比、提取次数及提取时间对狗脊总黄酮提取率的影响。结果最佳提取工艺为:60%乙醇、药液比1∶40、回流提取2次、每次1.5 h。结论在最佳工艺条件下,狗脊总黄酮的提取率为1.44%,高于其他条件下的提取率,该工艺条件可为狗脊总黄酮的工业生产提供依据。
Objective To optimize the extraction conditions for total flavonoids from Cibotium barometz by response surface method（RSM）.Methods According to the center combination of Box-Benhnken,using the RSM,the effects of ethanol concentration,the ratio of solid to liquid,the extraction time,and the extraction frequency were studied by central composite design.Results The optimal conditions of extraction were as follows：60% ethanol,the ratio of solid to liquid 1∶40,refluxing and extracting twice,and 1.5 h for each time.Conclusion The actual extraction yield was 1.44%.The method of extraction has higher extraction efficiency than other methods and can provide a basis for the industrial production of the total flavonoids from S.barometz.
出处
《国际药学研究杂志》
CSCD
北大核心
2017年第9期901-904,共4页
Journal of International Pharmaceutical Research
基金
陕西中医药大学科研基金项目(2016QN38)
关键词
金毛狗脊
总黄酮
提取工艺
响应面分析法
Cibotium barometz
total flavonoids
extraction technique
response surface method
