石墨炉原子吸收光谱法测定唾液中的锰

Detection of manganese in saliva with graphite furnace atomic absorption spectrometry

[目的]建立一种唾液中锰的石墨炉原子吸收分光光度检测方法。[方法]选取接触锰的234名工人为接触组,再将其分为高浓度接触组和低浓度接触组;选取不接触锰的135名工人作为对照组。采集唾液,离心,取0.5 mL上清液加硝酸消解,用体积分数0.2%硝酸定容至2 mL后,于原子吸收仪上测定。[结果]方法最佳灰化温度1100℃,最佳原子化温度1900℃,一次线性回归方程为Y=0.01587 x+0.00480,相关系数0.9992(P<0.01),特征浓度5.4 pg/0.0044 Abs,检出限为5.8 pg,加标回收率为85%~106%,相对标准偏差1.5%~1.8%。对照组工人唾液锰质量浓度均值(7.65±5.57)μg/L,接触组工人(10.63±12.37)μg/L,两组差异有统计学意义(P<0.01)。低浓度接触组唾液锰质量浓度(7.68±6.98)μg/L,高浓度接触组为(13.38±15.36)μg/L,两组均值差异有统计学意义(P<0.01)。[结论]该方法下不同锰接触水平人员的唾液中锰检测值不同,且该方法灵敏度高,准确度好,操作简便,故提示其可能适合于人体中锰的采样测定。

[Objective] To establish the graphite furnace atomic absorption spectrometry method to examine the manganese in saliva. [Methods] After centrifuge of saliva,0.5 mL of the supernatant was digested with nitric acid. 0.2% nitric acid was added to the constant volume 2 mL. The specimen was measured with atomic absorption spectrometer. Then samples of 234 welding workers and 135 control workers were detected. [Results] The best ashing temperature of the method was 1100 ℃,and the optimum atomic temperature was 1900 ℃. The linear regression equation was Y = 0.01587 x ＋ 0.00480 with the correlation coefficient 0.9992（P 〈0.01）. The characteristics of concentration was 5.4 pg/0.0044 Abs,and the detection limit was 5.8 pg.The recovery rate was 85%-106% with relative standard deviation 1.5%-1.8%. The average value of saliva manganese concentration was（7.65 ± 5.57） g/L and（10.63 ± 12.37） g/L in the control and welding workers,respectively（P 〈0.01）. The concentration of manganese varied among workers with lower or higher exposure level,（7.68 ± 6.98）g/L vs.（13.38 ＋ 15.36）g/L（P 〈0.01）. [Conclusion] The method is highly sensitive and accurate,easy to manipulate and well repeated. These suggested the method may be suitable for sampling and determination of manganese in human body.