弥漫性大B细胞淋巴瘤中C-MYC蛋白表达与基因异常的相关性

Correlation between C-MYC protein expression and genetic abnormalities in diffuse large B-cell lymphoma

目的探讨弥漫性大B细胞淋巴瘤（DLBCL）中癌基因C－MYC蛋白表达及与基因异常的相关性。方法回顾性收集山东省潍坊市人民医院病理科2015年1月至2016年10月首次诊断为DLBCL的石蜡包埋组织标本42例，采用免疫组织化学和荧光原位杂交（FISH）技术检测全部研究对象组织标本中C－MYC蛋白表达与基因异常的情况，观察两者之间的相关性。结果C－MYC蛋白表达阳性率为47.6%（20/42）；C－MYC基因异常检出率为26.2%（11/42），其中基因易位检出率23.8%（10/42）；基因扩增2.4%（1/42）；C－MYC蛋白表达阳性与C－MYC基因易位有相关性（χ2＝11.813；P＝0.001）；C－MYC基因易位主要见于生发中心B细胞型（χ2＝4.029；P＝0.045）。结论C－MYC蛋白表达阳性（≥40%）与C－MYC基因易位有相关性。在DLBCL中，C－MYC蛋白高表达提示可能存在C－MYC基因易位。

ObjectiveTo study the correlation between expression of oncogene C-MYC protein and gene abnormality in diffuse large B-cell lymphoma （DLBCL）.MethodsThe expression of C-MYC protein and gene abnormality were detected by immunohistochemistry and fluorescence in situ hybridization （FISH）, respectively, in 42 cases of paraffin-embedded DLBCL. All cases were collected at Department of Pathology, Weifang People′s Hospital during January 2015 to October 2016.ResultsThe positive rate of C-MYC protein expression was 47.6% （20/42） and the rate of abnormal C-MYC gene by FISH was 26.2%（11/42）, including translocation （23.8%, 10/42） and gene amplification （2.4%, 1/42）. There was a close relationship between the protein expression and gene translocation （χ2=11.813; P=0.001） and gene translocation occurred primarily in GCB （χ2=4.029; P=0.045）.ConclusionThe high expression （≥40%） of C-MYC protein is associated with its gene translocation, suggesting that C-MYC protein detection can be used as a surrogate marker for C-MYC gene translocation in DLBCL.