摘要
目的探讨弥漫性大B细胞淋巴瘤(DLBCL)中癌基因C-MYC蛋白表达及与基因异常的相关性。方法回顾性收集山东省潍坊市人民医院病理科2015年1月至2016年10月首次诊断为DLBCL的石蜡包埋组织标本42例,采用免疫组织化学和荧光原位杂交(FISH)技术检测全部研究对象组织标本中C-MYC蛋白表达与基因异常的情况,观察两者之间的相关性。结果C-MYC蛋白表达阳性率为47.6%(20/42);C-MYC基因异常检出率为26.2%(11/42),其中基因易位检出率23.8%(10/42);基因扩增2.4%(1/42);C-MYC蛋白表达阳性与C-MYC基因易位有相关性(χ2=11.813;P=0.001);C-MYC基因易位主要见于生发中心B细胞型(χ2=4.029;P=0.045)。结论C-MYC蛋白表达阳性(≥40%)与C-MYC基因易位有相关性。在DLBCL中,C-MYC蛋白高表达提示可能存在C-MYC基因易位。
ObjectiveTo study the correlation between expression of oncogene C-MYC protein and gene abnormality in diffuse large B-cell lymphoma (DLBCL).MethodsThe expression of C-MYC protein and gene abnormality were detected by immunohistochemistry and fluorescence in situ hybridization (FISH), respectively, in 42 cases of paraffin-embedded DLBCL. All cases were collected at Department of Pathology, Weifang People′s Hospital during January 2015 to October 2016.ResultsThe positive rate of C-MYC protein expression was 47.6% (20/42) and the rate of abnormal C-MYC gene by FISH was 26.2%(11/42), including translocation (23.8%, 10/42) and gene amplification (2.4%, 1/42). There was a close relationship between the protein expression and gene translocation (χ2=11.813; P=0.001) and gene translocation occurred primarily in GCB (χ2=4.029; P=0.045).ConclusionThe high expression (≥40%) of C-MYC protein is associated with its gene translocation, suggesting that C-MYC protein detection can be used as a surrogate marker for C-MYC gene translocation in DLBCL.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2018年第3期172-175,共4页
Chinese Journal of Pathology
基金
山东省科技攻关项目(2015GSF118168)
潍坊市发展计划课题(20100220)