胆汁外引流对重症急性胰腺炎大鼠肺部炎症和水肿的疗效及机制研究

Effect of external biliary drainage on lung inflammation and edema in severe acute pancreatitis rats

目的 :探讨胆汁外引流对重症急性胰腺炎大鼠肺部炎症和水肿的疗效与机制。方法 :24只雄性SpragueDawley(SD)大鼠随机分为4组(n=6):假手术组(SS组)、重症急性胰腺炎组(SAP组)、SAP+胆汁外引流组(SAP+BTED组)、SAP+BTED+锌原卟啉组(SAP+BTED+Zn PP组)。Zn PP是血红蛋白氧合酶1(HO-1)的特异性阻断剂,抑制HO-1的表达。造模24 h后取肺组织和血液备用。采用Schmidt评分标准对肺组织进行病理评分。采用Western印迹法检测肺组织中HO-1蛋白质水平表达。采用荧光定量RT-PCR法检测肺组织中HO-1、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)m RNA基因水平的表达。采用ELISA法检测肺组织中髓过氧化物酶(MPO)的含量。计算肺组织干湿比。结果:SAP+BTED组大鼠肺组织Schmidt评分显著低于SAP组大鼠(P<0.05)。BTED使肺组织中TNF-α和IL-6 m RNA基因表达水平、MPO含量显著低于SAP组大鼠(P<0.05)。SAP+BTED组大鼠干湿比和HO-1表达水平显著高于SAP组大鼠(P<0.05)。当应用Zn PP时,BTED引起大鼠的这些效应则消失。结论:BTED通过HO-1通路减轻SAP大鼠肺部的炎症和水肿。

Objective To investigate the roles of biliary tract external drainage（BTED） on lung inflammation and ede- ma of the rats with severe acute panereatitis （SAP）. Methods Twenty-four SD rats were randomly divided into four groups of six each： group sham surgery（SS）, group SAP, group SAP＋BTED with the SAP rats established first and then BTED per- formed, group SAP＋BTED＋zine protoporphyrin （ZnPP） with Znpp injected abdominally 24 h before and then SAP with BTED procedure. ZnPP is a specific inhibitor of heine oxygenase-1（HO-l）, which can inhibit the expression of HO-I. The rats were sacrificed 24 h after surgmy. The lung and blood were collected. The pathological changes of lung were scored by Schmidt criterion, mRNA expression of HO-1, TNT-α and IL-6 were detected by quantitative RT-PCR lnethod, hmg HO-1 expression by Western blot and myeloperoxidase （MPO） protein by ELISA. The ratio of dry/wet of lung tissue was calculated. Results The Schmidt score in group SAP＋BTED were significantly lower than in group SAP（P〈0.05）. The ex- pression of both TNT-α and IL-6 with MPO level in group SAP＋BTED were significantly lower than in group SAP（P〈0.05）. But the dry/wet ratio and the expression of HO-1 protein in group SAP＋BTED were significantly increased than in group SAP（P〈0.05）. All these effects induced by BTED were not present when ZnPP war given. Conclusions BTED could reduce the lung inflammation and edema of the rats with SAP via the HO-I pathway.