皮秒脉冲电场调控大鼠运动皮层神经元spike发放的初步研究

Preliminary Study on the Regulation of Spike Release in Motor Cortical Neurons of Rats by Picosecond Pulsed Electric Field

近年来,皮秒脉冲电场诱导的生物电效应逐渐引起了国内外研究学者的关注。现有细胞实验已经初步证实,皮秒脉冲电场对神经元细胞及其动作电位有一定影响。然而,目前国内外尚未见皮秒脉冲对神经元spike发放影响的相关研究报道,皮秒脉冲对动物神经元spike发放的调控效果仍为一个未知数。为此,基于可调式皮秒脉冲发生器（最大峰值电压–1 000 V,半高宽550 ps,上升时间150 ps,重复频率0~10 k Hz可调）,以大鼠运动皮层神经元为实验对象,有效开展了皮秒脉冲对大鼠运动皮层电生理活动影响的研究,初步探讨了皮秒脉冲幅值、重复频率及作用时间等对大鼠运动皮层神经元spike发放的影响。实验发现：大鼠运动皮层神经元spike发放的改变与皮秒脉冲幅值、重复频率及作用时间密切相关。当皮秒脉冲电压幅值绝对值大于1 k V时,神经元spike发放开始出现变化。随着皮秒脉冲重复频率的增大,神经元spike发放有先增强后抑制的趋势。随着皮秒脉冲作用时间的增加,神经元spike发放出现增强或抑制效果的频率响应窗口均提前。

In recent years, the bioelectric effect induced by picosecond pulsed electric field has gradually elicited the interest of numerous researchers at home and abroad. The previous cell experiments have confirmed that picosecond pulsed electric field has influences on the neuron cell and its action potential. However, there is no report about the effect of picosecond pulse on the spike release of neurons. The effect of picosecond pulse on the regulation of spike release in animal neurons remains unknown. So, we use an adjustable picosecond pulse generator（maximum peak voltage of-1 000 V, full width at half maximum（FWHM） of 550 ps, rise time of 150 ps, and repetition frequency of 0~10 k Hz） as an experimental device, and use the rat cortex neurons as experimental subjects to investigate the effect on electrophysiological activity of motor cortex in rats by picosecond pulse. The effects of picosecond pulse amplitude, repetition frequency, and time on spike release of motor cortical neurons in rats are preliminarily studied. Based on this experiment, we find that the spike release in motor cortical neurons of rats is related to the amplitude, repetition frequency, and duration of picosecond pulse. When the absolute value of the picosecond pulse voltage is greater than 1 k V, the spike release in neurons begins to change. With the increase of picosecond pulse repetition rate, the spike release in neurons enhances first and then inhibits. With the increase of the picosecond pulse time, the frequency response window of the enhancement and inhibition of the spike release in neurons is advanced.