基于网格传输网络模型的纳秒脉冲作用下单细胞电穿孔规律仿真研究

Simulation Study on Electroporation Law in a Single-cell System Exposed to Nanosecond Pulsed Electric Fields Based on Mesh Transport Network Model

为研究ns脉冲电场杀伤肿瘤细胞的作用机理,采用MATLAB仿真软件对含细胞核膜的典型单细胞空间区域进行了离散,建立了其网格传输网络模型,分别从空间电位分布、跨膜电压曲线以及孔数密度、孔半径分布等角度,仿真模拟了单细胞在电场强度为0.5 MV/m、脉冲宽度为250 ns、上升沿为30 ns、仿真总时长为1μs的ns脉冲作用下的电穿孔动态变化过程。仿真研究结果表明：无论是细胞膜还是细胞核膜,电穿孔都存在＂负极性效应＂,即总是负极正对膜位置先出现电穿孔;细胞膜电穿孔存在较明显的＂过渡区域＂,且过渡区域孔半径较大,孔数密度较小;细胞膜电穿孔比细胞核膜更加容易。该仿真研究结果丰富了脉冲电场用应于治疗肿瘤领域的电穿孔机理,为进一步研究高频ns脉冲串的电穿孔机理提供了仿真思路。

In order to study the lethality mechanism of tumor cells exposed to high-frequency nanosecond pulse bursts, a typical single cell system including cell nuclear membrane was discretized into nodes with the help of MATLAB, and the mesh transport network method model was established the dynamic process in single-cell electroporation was simulated and analyzed when applying one nanosecond pulse（electric field： 0.5 MV/m, pulse width： 250 ns, rising edge time ： 30 ns, total simulation time ： 1 μs） to the targeted system. The results show that whether the cell membrane or cell nuclear membrane, the electroporation has its ＂negative polarity effect＂, that is the electroporation always happen at the membrane facing cathode. In addition, there is a transition region on the cell membrane for electroporation,where the radius of pore is larger but the density is smaller than other positons. Comparing with the cell nuclear membrane, the electroporation of cell membrane is much more easier. The simulation results of this paper enrich the electroporation mechanism of pulsed electric field acting on the biological cells, and provide a simulation model idea for further study of the electroporation law of high frequency nanosecond pulsed bursts.