罗布麻内生细菌的分离鉴定及抑菌活性

Isolation and identification of endophytic bacteria from Apocynum venetum L.and antibacterial activity

目的:研究罗布麻内生细菌及其抑菌活性,旨在深入开发新资源。方法:以红花罗布麻为研究对象,采用平板分离法从中分离内生细菌,利用牛津杯法对其发酵物进行抑菌活性研究,对有抑菌活性的菌株进行形态特征观察和16S rRNA序列同源性分析。结果:筛选出的D1菌株对大肠杆菌、金黄色葡萄球菌、藤黄微球菌和枯草芽孢杆菌四株供试指示菌均有明显的抑制作用,D3菌株对藤黄微球菌和枯草芽孢杆菌有一定的抑菌作用。结合菌株的形态学特征和16S rRNA序列同源性分析,将D1鉴定为假单胞菌,D3鉴定为菠萝泛菌。结论:罗布麻中蕴含着具有抑菌活性的内生细菌资源,可进一步的开发利用。

Objective：To study the endophytic bacteria from Apocynum venetum L.and their antibacterial activity in order to find novel resources.Methods：Endophytic bacteria were isolated from Apocynum venetum L.by using the method of plate cuhivation, and the antibacterial activity of the fermented metabolites was studied by the Oxford Cup method. Meanwhile, the target endophytic bacteria were identified by 16S rRNA sequence homologous analysis combined with their physiological and biochemical characteristics.Resuhs：The fermentation broths of endophytic bacteria showed different antibacterial activity.The strain D1 had inhibitory effect on Escherichia coli, Staphylococcus aureus, Micrococcus lutea and Bacillus subtilis, the strain D3 had inhibitory effect on Micrococcus lutea and Bacillus subtilis.The D1 was identified to be Pseudornonas hananensis, and D3 was identified to be Pantoea ananatis. Conclusion： The results showed that Apocynum venetum L. contains abundant endophytic bacteria resources with antibacterial activity, which could be further developed and utilized.