响应面法优化三种天然产物对黄嘌呤氧化酶的抑制作用

Optimization of the three natural products' inhibitory effect on xanthine oxidase by response surface methodology

选取槲皮素、没食子酸和葛根素三种天然产物作为研究对象,测定三种天然产物对黄嘌呤氧化酶的抑制作用及酶抑制剂作用类型。在单因素实验基础上,以对黄嘌呤氧化酶的抑制率为响应值,应用响应面法对三种天然产物进行复配优化。结果显示,槲皮素、没食子酸和葛根素溶液对黄嘌呤氧化酶抑制的IC_(50)分别为44.86、183.61和555.20μg/mL,抑制效果为:槲皮素>没食子酸>葛根素,且均为可逆反应。响应面模型可靠,最优复配浓度为:槲皮素83.25μg/mL,没食子酸166.50μg/mL,葛根素650.00μg/mL,对黄嘌呤氧化酶抑制率为96.92%±0.17%,与模型预测抑制率96.97%极为接近。本实验不仅减少抑制剂的用量,提高效率,而且为开发利用天然产物及抗痛风食品研发奠定基础。

Quercetin, gallic acid and puerarin were selected as the objects of this study.Inhibitory rates and reaction types of the three natural products on xanthine oxidase were studied first.The method of response surface methodology was applied to study the three nature products＇ inhibitory rate on xanthine oxidase based on single-factor tests. The results showed that the three nature products were reversible inhibitors against xanthine oxidase and the IC50 of quercetin, gallic acid and puerarin were 44.86 μg/mL, 183.61 μg/ulL and 555.20 μg/mL, respectively.The inhibitory effect of quercetin was better than gallic acid and puerarln,and the inhibitory effect of puerarin was the worst. The results showed that the response surface methodology was reliable, and the optimal extraction conditions were 83.25 μg/mL for quercetin, 166.50 μg/mL for gallic acid, and 650.00 μg/ml, for puerarin.In this case,the inhibitory rate was 96.92% _＋0.17% ,was very closed to the model predicts inhibition rate 96.97%. One side this study reduced the dosage and improved the efficiency of the inhibitors, on the other side, it laid foundation for natural products development and food research on anti-gout.