鱼精蛋白结合壳聚糖对草鱼片的冷藏保鲜效果

Effect of protamine and chitosan mixture on fresh preservation of Ctenopharyngodon idellus

为探讨鱼精蛋白-壳聚糖复合保鲜剂对草鱼贮藏保鲜的可行性,本研究采用3种不同复合比例处理草鱼,4℃冷藏期间定期测定其菌落总数、TVB-N、汁液流失率、pH、鲜度指标K值并进行感官评价,反应鱼肉品质变化情况。结果表明:复合处理组(CP1、CP2、CP3)贮藏期间感官评分均显著高于对照组(CK),但处理组对pH变化影响较小。各组感官评分值与菌落总数、TVB-N、汁液流失率、K值变化趋势均表现出极显著相关性(p<0.01),相关系数r在-0.887^-0.991之间。其中CP2处理组(1%壳聚糖+0.5%鱼精蛋白溶液)保鲜效果最佳,贮藏10 d各指标均在可接受范围,相比对照组至少延长保鲜期4 d以上。这表明壳聚糖-鱼精蛋白复合保鲜剂能在贮藏期间较好的保持草鱼品质,延长其货架期。

In order to investigate the feasibility of protamine-chitosan composite preservative on storage of Ctenopharyngodon idellus ,the grass carp fillets were processed by 3 different composite ratios and stored at 4 ℃ .Indexes including Total viable counts（TVC） ,Total volatile base nitrogen（ TVB-N）, drip loss, pH, freshness index K value and sensory evaluation value were evaluated to reflect the changes of fish quality.The results showed that the composite treatment groups （CP1, CP2, CP3 ）had distinctly higher sensory scores than the control group （CK） during storage, while as with pH, no obvious difference was observed.The variation trend of sensory evaluation showed a highly significant correlation with tendency variation of TVC, TVB-N,drip loss and K value （p 〈 0.01 ）, the correlation coefficient r lying in -0.887～ -0.991.The results indicated that protamine-chitosan composite preservative could better maintain the quality of grass carp during storage time and prolong its shelf life.Above all, the C P2 treatment group （ 1% ＋ 0.5 % chitosan protamine solution）possessed the best fresh-keeping effect, which could be preserved for 10 days with each index beingin the acceptable range, extending the shelf life for more than 4 days compared with the control group.