摘要
The gastrointestinal tract, as the major digestive and absorbing organ, plays an important role in chicken growth. Numerous studies have been undertaken to investigate the gut bacterial community in broilers, but little is known about the archaeal community composition. To improve our understanding of the composition of intestinal archaea and the relationship between archaea and chicken growth, a feeding trial was conducted with 10 000 Ross 308 chicks in a commercial production facility under standard management. At 38 d of age, 15 birds with the highest body weight （ H group）, 15 birds with average body weight （ M group）, and 15 birds with the lowest body weight （ L group） were selected among 600 healthy male individuals. Cecal archaeal community was detected based on 16S rRNA gene sequencing. As a result, 3 321 229 archaeal sequences were clustered into 1 281 operational taxonomic units （OTUs） and classified into three phyla （Crenarchaeola, Euryarchaeota and Thaumarchaeota） and six known genera ： Natronomonas, Halolamina, Salinarchaeum, Cenarchaeum, Candidatus, Nitrosoarchaeum and Nitrososphaera. The alpha indices showed that, the richness of archaea in L group was the highest, and followed by that in M group and H group, while the archaea in H group were more diverse, followed by those in M group and L group. However, the archaeal composition in all chicken samples was similar, with the vast majority of the sequences belonging to Cenarchaeum, and only a few belonging to the other five genera.
The gastrointestinal tract, as the major digestive and absorbing organ, plays an important role in chicken growth. Numerous studies have been undertaken to investigate the gut bacterial community in broilers, but little is known about the archaeal community composition. To improve our understanding of the composition of intestinal archaea and the relationship between archaea and chicken growth, a feeding trial was conducted with 10 000 Ross 308 chicks in a commercial production facility under standard management. At 38 d of age, 15 birds with the highest body weight （ H group）, 15 birds with average body weight （ M group）, and 15 birds with the lowest body weight （ L group） were selected among 600 healthy male individuals. Cecal archaeal community was detected based on 16S rRNA gene sequencing. As a result, 3 321 229 archaeal sequences were clustered into 1 281 operational taxonomic units （OTUs） and classified into three phyla （Crenarchaeola, Euryarchaeota and Thaumarchaeota） and six known genera ： Natronomonas, Halolamina, Salinarchaeum, Cenarchaeum, Candidatus, Nitrosoarchaeum and Nitrososphaera. The alpha indices showed that, the richness of archaea in L group was the highest, and followed by that in M group and H group, while the archaea in H group were more diverse, followed by those in M group and L group. However, the archaeal composition in all chicken samples was similar, with the vast majority of the sequences belonging to Cenarchaeum, and only a few belonging to the other five genera.
基金
Supported by the Special Fund for Public Projects of Zhejiang Province(2016C32073)
Agriculture Project of Guizhou Province(20103047)
International Cooperation Program of Zhejiang Academy of Agricultural Sciences
