四君子汤合血府逐瘀汤对利血平胃溃疡模型小鼠胃黏膜、胃组织SOD、MDA、NO及气、血、阴盛衰影响随机平行对照研究

Randomized Parallel Contrast Study of Effects of Sijunzi Decoction plus Xuefuzhuyu Decoction on the Gastric Mucosa, Gastric SOD, MDA, NO and Exuberance and Debilitation of Qi, Blood and Yin, in Reserpine Induced Gastric Ulcer Model Mice

[目的]观测四君子汤合血府逐瘀汤对利血平胃溃疡模型小鼠胃黏膜、胃组织SOD、MDA、NO及气、血、阴盛衰影响。[方法]使用随机平行对照方法,将40只昆明种小鼠按按随机数字表分为4组,空白对照组、模型组、奥美拉唑组、中药复方组,10只/组。参照利血平胃溃疡模型小鼠制备方法复制胃溃疡模型,实验第8d除空白对照组外,3组均皮下注射利血平0.1mg/10g。实验第9d药物干预,奥美拉唑组腹腔注射,中药复方组四君子汤合血府逐瘀汤灌胃干预,连续5d,1次/d。行旷场试验,抓力检测,并测定爪/尾r值,计算各组小鼠气、血和阴盛衰,检测胃黏膜、胃组织SOD、MDA、NO含量。[结果]实验第13d模型组胃黏膜多见较大面积溃疡。气、血、阴盛衰度:实验第7d各组均无显著差异(P>0.05),实验第9d模型组、奥美拉唑组、中药复方组均低于空白对照组(P<0.01),实验第13d(干预第5d)中药复方组与奥美拉唑组干预组均改善(P<0.01,P<0.05),组间无显著差异(P>0.05)。溃疡指数:实验第13d(药物干预第5d)奥美拉唑组、中药复方组均低于模型组(P<0.01),中药复方组与奥美拉唑组无显著差异(P>0.05)。SOD、MDA、NO:实验第13d,SOD模型组、中药复方组均低于空白对照组(P<0.01),奥美拉唑组与空白对照组无显著差异(P>0.05),奥美拉唑组、中药复方组均高于模型组(P<0.01),中药复方组低于奥美拉唑组(P<0.01);MDA模型组、奥美拉唑组、中药复方组均高于空白对照组(P<0.01),奥美拉唑组、中药复方组均低于模型组(P<0.01),中药复方组高于奥美拉唑组(P<0.01);NO模型组、奥美拉唑组、中药复方组均低于空白对照组(P<0.01),奥美拉唑组低于模型组(P<0.01),中药复方组与模型组、奥美拉唑组无显著差异(P>0.05)。[结论]四君子汤合血府逐瘀汤可改善利血平胃溃疡模型小鼠气血两虚,降低溃疡指数,提高溃疡抑制率,增加组织SOD活力与NO含量,降低MDA含量。

[Objective] To study the effects of Sijunzi decoction plus Xuefuzhuyu decoction on the gastric mucosa, gastric SOD, MDA, NO and exuberance and debilitation of Qi, blood and Yin, in reserpine induced gastric ulcer model mice. [Method] By using random parallel control methods, the 40 kunming mice were divided into four groups of random numbers, and the control group, the model group, the olometriazole group, the Chinese herbal remedy group, 10/group. The gastric ulcer model was replicated with the mousepreparation method of lihematine gastric ulcer model. To replicate the ulcer model of the gastric ulcer with reserpine. In the 8 th day of the experiment, except the blank control group, the other three groups were injected with reserpine（0.1 mg/10 g, subcutaneous injection）. Drug intervention began on the 9 th day of the experiment. The Omeprazole group is given an intraperitoneal injection. the Chinese herbal remedy group is given to fill the stomach by Sijunzi decoction plus Xuefuzhuyu decoction. Continuous 5 days, once a day.To conduct the field test and the determination of grasping force to measure the value of claw/tail r, and to calculate the exuberance and debilitation of Qi, blood and Yin, the SOD, MDA, and NO. [Result] On the 13 th day of the experiment, the gastric mucosa of the model group showed a large number of ulcers. Xuberance and debilitation of Qi, Blood and Yin： On the 7 th day, there was no significant difference between the groups（P0.05）. In the 9 th day, the model group, the omeprazole group and the Chinese herbal remedy group were lower than the blank control group（P0.01）. In the 13 th day of the experiment（intervention 5）, the intervention groups of the Chinese herbal remedy group and omeprazole were all improved（P0.01, P0.05）. There was no significant difference between the groups（P0.05）. Ulcer index： There was no significant difference between the Chinese herbal remedy group and omeprazole group（P0.01）, and the Chinese herbal remedy group was lower than that in the model group（P0.01）. SOD, MDA, NO： On the 13 th day of the experiment,SOD of the model group and Chinese herbal remedy group were lower than the blank control group（P0.01）.There was no significant difference between omeprazole group and blank control group（P0.05）. Both the omeprazole group and the Chinese herbal remedy group were higher than the model group（P0.01）. The Chinese herbal remedy group was lower than omeprazole（P0.01）. MDA of the model group, omeprazole group and the Chinese herbal remedy group were higher than the blank control group（P0.01）. Both the omeprazole group and the Chinese medicine compound group were lower than the model group（P0.01）.The Chinese herbal remedy group was higher than omeprazole（P0.01）. NO of the model group, the Chinese herbal remedy group, the omeprazole group were lower than blank control group（P0.01）, omeprazole group is lower than the model group（P0.01）, the compound Chinese medicine group and model group, omeprazole group NO significant difference（P0.05）. [Conclusion] Sijunzi decoction plus Xuefuzhuyu decoction can improve Gas and Blood deficiency of reserpine gastric ulcer model mice, reduce ulcer index, improve the ulcer inhibition rate, increase the tissue SOD activity and NO content, reduce the MDA content.