苍术炒焦前后正丁醇部位对脾虚泄泻证大鼠的药效研究

Pharmacodynamics of n-butyl alcohol parts extracted fromAtractylodis lancea before and after the deep-fired in model rats with spenasthenic diarrhea

目的:通过比较苍术炒焦前后正丁醇提取部位对脾虚泄泻证大鼠的药效变化,阐释焦苍术健脾止泻机制。方法:以脾虚泄泻证大鼠为模型,测定不同组别大鼠胃残留率、肠推进率、血清胃动素(MTL)水平、胃泌素(GAS)水平、结肠黏膜水孔蛋白3(APQ_3)表达水平及血清肿瘤坏死因子(TNF-α)、白细胞介素10(IL-10)的水平。结果:经统计学分析,生品组和焦品组各项指标均较模型组有不同程度的改善,其中焦品中、高剂量组大鼠胃残留率、腹泻指数和血清TNF-α水平显著降低,小肠推进率、结肠APQ_3及血清MTL、GAS、IL-10水平显著升高;并与正常组和阳性对照组比较无显著性差异;而与相同剂量生品组比较具有显著性差异。结论:苍术炒焦后正丁醇提取部位健脾、止泻、抗炎作用增强,正丁醇部位是焦苍术"健脾止泻"的主要有效部位之一。

OBJECTIVE To compare the change of efficacy of n-butyl alcohol parts extracted fromAtractylodis lancea before and after deep-fired in model rats with spenasthenic diarrhea,interpret the mechanism of spleen antidiarrheal about the focal Rhizoma Atractylodis.METHODS With rats with diarrhea pixu（spleen deficient）as the test models,gastric residual rate,intestine propulsive rate,the MTL level,the GAS level,colonic mucous membrane hole protein 3（APQ_3）expression levels and serum tumor necrosis factor（TNF-α）,the level of interleukin 10（IL-10）were determined.RESULTS By using the raw and fired herbs,all the indicators had different degrees of improvement in the model group,but the gastric residual rate,diarrhea index and the level of serum TNF-αwere reduced obviously in the middle and high dose groups of rats.Compared with normal control group,positive control group showed no statistical difference.However,it has significant difference between the fired product groups and the same dose of raw product groups.CONCLUSION After Rhizoma Atractylodis is deep-fried,the functions about strengthening the spleen,checking diarrhea and anti-inflammation are enhanced,which confirms that n-butyl alcohol part is the mainly effective part for spleen antidiarrheal effect.