转录组测序研究三角帆蚌珍珠颜色相关基因

Transcriptome sequencing analysis on pearl color related genes in Hyriopsis cumingii

为筛选影响三角帆蚌珍珠颜色的候选基因,采用转录组测序平台Illumina Hi Seq TM2500对蚌壳珍珠层颜色为紫色和白色的三角帆蚌中央膜组织进行高通量测序。所得序列经质控、组装后比对到NR、Swiss-Prot、COG、KOG、KEGG、GO和Pfam数据库中注释,并进行差异基因聚类分析。结果显示,获得干净数据共72 800 581 bp,拼接获得了89 529个Unigene,差异表达基因2644个,其中上调表达基因1323个,下调表达基因1321个。根据GO功能分类可分为分子功能、细胞组分、生物过程等3大类50分支;根据KEGG代谢通路分析可以分为187类。对差异表达基因分析发现,部分基因与色素合成(眼黄素、黑色素、喋啶色素)及金属离子转运相关,还有6个细胞色素P450,3个细胞色素b561和1个细胞色素b560。研究表明,这些基因可能在珍珠颜色形成中发挥作用。

The transcriptome of Hyriopsis cumingii ventral mantle tissue whose nacre colors were purple and white was sequenced by a new generation of high throughput sequencing technology in order to find candidate genes involved in pearl color produced by H. cumingii. After quality control and assembly, the sequences acquired were blasted against NR, Swiss-Prot, COG, KOG, KEGG, GO and Pfam databases, and then cluster analysis were performed. In total, 72 800 581 bp of clean data, and 89 529 Unigenes were obtained, and 2644 genes were differentially expressed, among which 1323 were up-regulated and 1321 down-regulated. The Unigenes GO function in the transcriptome library was divided into 3 categories： molecular function, cellular component and biological process with 50 branches; it could be divided into 187 classes taking the KEGG database as a reference. Differentially expressed gene analysis revealed that some genes were related to pigment biosynthesis（such as xanthommatin,melanin and pteridine） and metal ion transportation. And there were 6 cytochrome P450, 3 cytochrome b561 and 1 cytochrome b560 genes. They might play important roles in the formation of pearl color of H. cumingii.