摘要
目的:探讨胰升血糖素样肽-1(GLP-1)类似物利拉鲁肽(LRG)对犬骨髓间充质干细胞(BMSCs)成骨分化的影响及相关的信号机制。方法:分离、培养犬BMSCs,MTT法检测不同浓度LRG对BMSCs增殖的影响,碱性磷酸酶(ALP)活性检测筛选最佳的LRG干预浓度,实时荧光定量逆转录PCR(qRT-PCR)检测成骨相关基因R unx 2、COL-I和OCN的表达,Wester n blot检测PI3K、Ak t的磷酸化水平。结果:不同浓度的LRG对BMSCs的生长曲线无影响;LRG呈浓度依赖性地增强ALP活性;LRG显著上调Runx2和COL-I的表达(P<0.05),OCN的表达也呈上升趋势;LR G显著提高PI3K和Ak t的磷酸化水平(P<0.05)。结论:LRG可通过激活PI3K/Ak t信号通路促进BMSCs骨向分化。
Objective： To investigate the effects of liraglutide （LRG）, a type of glucagon-like peptide-1 analogs, on osteoblast differentiation of dog-derived BMSCs and related signaling mechanisms. Methods： BMSCs of dogs were extracted and cultured. The growth curves in different dose of LRG administration were measured by MTT method. After cultured in osteogenic differentiation medium, ALP activity was tested to choose the optimum dose of LRG administration, qRT-PCR was used to evaluate the expression levels of Runx2 、 COL-I and OCN, phosphorylation levels of PI3K and Akt were detected by Western blot. Results： Different dose of LRG had no influence on the growth ofBMSCs; LRG promoted ALP activity in a dose-dependent manner; LRG could significantly up-regulate mRNA expression of Runx2 and COL-I （P〈 0.05）, trended to elevate OCN mRNA levels; phosphorylation levels of PI3K and Akt significantly increased by LRG intervention （P〈 0.05）. Conclusion： LRG promotes osteoblast differentiation of BMSCs by activating PI3K/Akt signaling pathway.
出处
《口腔颌面修复学杂志》
2018年第1期44-47,共4页
Chinese Journal of Prosthodontics
基金
国家自然科学基金(项目编号:81570730)
