心脏侧群细胞在体外的增殖与分化

Proliferation and differentiation of cardiac side population cells in vitro

目的:描述成年小鼠心脏的SCA1-/CD31-CSP细胞特征,研究它们在体外的增殖、分化能力。方法:通过荧光激活细胞(流式细胞仪)、逆转录酶聚合酶链反应、甲基纤维素和内皮细胞培养基培养,对细胞进行分选、基因、增殖、分化的检测。结果:SCA1-/CD31-CSP细胞表达干细胞和内皮祖细胞基因,这些细胞能够在体外增殖、分化和血管化。结论:在鼠的心脏中SCA1-/CD31-CSP细胞可作为内皮祖细胞。

Objective：To describe feature of SCA1-/CD31-CSP cells in the adult mouse heat investigated their abilities of proliferation and differentiation in vitro.Method：Using fluorescence-activated cell sorting and reverse transcriptase polymerase chain reaction,we detected genes,proliferation,differentiation in Methyl cellulose and endothelial cells medium.Result：The results showed that SCA1-/CD31-CSP cells express stem cell and endothelial progenitor genes.These cells were capable of proliferation,differentiation and vascularization in vitro.Conclusion：SCA1-/CD31-CSP cells may serve as endothelial progenitor cells in the mouse heart.