江夏地区人群RhD(-)血型系统基因频率的分布

Gene frequency distribution of RhD negative blood group system in Jiangxia area

目的:探讨RhD阴性血型基因及其MN、P血型基因在江夏地区人群中的分布频率。方法:采用血清学方法对江夏区42 940例住院患者及健康体检者进行RhD、C、c、E、e、M、N、P1抗原表现型检测。结果:共检出RhD阴性136例,其中ccee 76例(50.88%),CCee 10例(7.35%),Ccee 42例(30.88%),ccEe 6例(4.41%),CcEe 2例(1.47%)。从而得出RhD阴性者中C基因频率为0.235 3,c基因频率为0.764 7,E基因频率为0.029 4,e基因频率为0.706,基因组合体频率为cde=0.042,Cde=0.012 6,cdE=0.001 7,CdE=0,并对M、N及P1抗原进行了分布调查,其中M型34例(25%),N型64例(47.06%),MN型38例(27.94%),P1型50例(36.76%)。结论:RhD阴性表型按频率由高到低依次为deecc>deeCc>deeCC>dEecc>dEeCc,由单倍型来看cde是江夏地区RhD阴性中最常见单倍型,Cde次之,结果比较符合中国汉族RhD阴性血型中Rh单倍型和基因频率分布状况。同时对RhD阴性血的MN及P系统有初步的了解。

Objective：To explore the distribution of RhD negative and MN/P blood groups among Jiangxia district in Wuhan Method：A total of 42 940 inpatient and healthy individuals were tested for RhD,C,c,E,e,M,N and P1 phenotype by serological experimental in Frist Renming Hospital of Jiangxia district.Result：It was totally detected 136 D-negative blood samples,among which were 5 Rh phenotypes：76 cases of ccee（50.88%）,10 cases of Ccee（7.35%）,42 cases of Ccee（30.88%）,6 cases of ccEe（4.41%）,and 2 cases of CcEe（1.47%）.Among RhD negative individuals,gene frequencies for C,c,E,and e were 0.235 3,0.764 7,0.029 4 and0.706,respectively.cde,Cde,cdE and CdE chromosome frequencies were 0.042,0.012 6,0.001 7 and nearly0,respectively.M,N and P1 antigen distribution investigation displayed that 34 cases of M（25%）,64 cases of N（47.06%）,38 cases of MN（27.94%）and 50 cases of P1（36.76%）.Conclusion：The frequency of RhD negative phenotype from high to low was deecc,deeCc,deeCC,dEecc and dEeCc.Among them cde was the most haplotype in Jiangxia district,and Cde came second.The distribution feature of Rh haplotype and gene frequency among Jiangxia were similar to those among Han population in China.And we had a first impression of MN and P blood group system in RhD negative.