低频抗-Mur抗体鉴定及血型特征研究

Low frequency of anti-Mur caused by incompatible blood in one case

目的:通过对1例冰冻保存的抗筛阴性、交叉配血不合的献血者标本进行检测分析,了解低频血型抗体类型和血型特征。方法:采用不同厂家的抗体筛选细胞对冰冻保存标本和新采集标本进行抗体检测和鉴定,并检测抗体效价;采用多种血型血清学方法检测抗-Mur抗体与Mur抗原的红细胞凝集反应特征。结果:该献血者标本与具有Mur抗原的筛选细胞血清凝集反应阳性,与其余筛选细胞反应均为阴性;抗体鉴定结果为IgG抗-Mur抗体;冰冻保存标本和新采集标本的抗体效价均为16;Mur抗原抗体在酶介质中不凝集。结论:导致抗筛阴性、交叉配血不合的不规则抗体为IgG抗-Mur抗体,IgG抗-Mur抗体冰冻保存效价比较稳定且可在人体内较长时间稳定存在,Mur抗原抗体反应符合MN血型抗原抗体血型血清学凝集反应特征。

Objective：To investigate the types and characteristics of low frequency blood group antibody in 1 case of frozen blood samples with negative screening and incompatible cross matching.Method：The antibodies of frozen specimens and fresh specimens were detected and identified by antibody screening cell of different manufacturers,and antibody titer was detected;red cell agglutination characteristics of anti-Mur antibody and Mur antigen were detected using a variety of serological method.Result：The serum agglutination reaction of specimen of the blood donor with Mur antigen positive cells were positive,and with other screening cells were negative.The antibody identification result was IgG anti-Mur antibody.The antibody titer of frozen preserved specimens and collected samples were 16.Mur antigen and antibody was not agglutinated in the enzyme medium.Conclusion：The irregular antibodies that caused negative screening and incompatible cross matching were IgG anti-Mur antibody,and the anti-Mur antibody of IgG could be stable in human body for a long time.Mur antigen and antibody reaction accorded with serological agglutination reaction characteristics of MN blood group.