白藜芦醇对脑小胶质细胞缺氧损伤的保护机制研究

Protective mechanism of Resveratrol on hypoxia injury of brain microglia

目的:研究白藜芦醇对脑小胶质细胞缺氧性损伤的保护作用。方法:使用不同浓度(0μmol/L、12.5μmol/L、25μmol/L、50μmol/L)的白藜芦醇干预脑小胶质细胞缺氧模型,通过细胞生长曲线及克隆形成实验,检测白藜芦醇对缺氧脑小胶质细胞存活率的影响。结果:白藜芦醇作用2 d后,各浓度组脑小胶质细胞存活率与缺氧组比较,差异均无统计学意义(均P>0.05)。白藜芦醇作用4 d后,随着药物浓度的增加,缺氧脑小胶质细胞存活率分别为(34.17±8.25)%、(55.20±7.55)%、(78.13±6.85)%、(90.07±7.84)%,组间比较差异有统计学意义(均P<0.001)。作用6d后,缺氧脑小胶质细胞存活率分别为(39.01±5.54)%、(60.08±7.13)%、(80.37±5.31)%、(92.03±7.62)%,组间比较差异有统计学意义(均P<0.001)。脑小胶质细胞克隆存活率随白藜芦醇浓度增加而增高。随药物浓度增加,白藜芦醇干预7 d后,克隆存活率分别为(26.47±5.66)%、(47.92±5.07)%、(66.09±2.93)%、(80.07±1.49)%,组间比较差异有统计学意义(均P<0.001)。结论:白藜芦醇可以提高在缺氧条件下脑小胶质细胞的存活率。

Objective：To study the protective effect of resveratrol on hypoxic injury of brain microglia. Methods：The hypoxia model of microglia was treated with resveratrol at different concentrations（0,12. 5,25,and 50 μmol/L）. The cell growth curve experiment and clone formation experiment were used to check up the effect of resveratrol on the survival of brain microglia hypoxia model. Results：As compared with the hypoxia group,the low dose group,middle dose group and high dose group showed no significant difference in the survival rate of MG cells after treatment with resveratrol for 2 days（P〈0.05）. With the increase of drug concentration,the survival rates of hypoxia model were（34. 17 ± 8. 25） %,（55. 20 ±7. 55） %,（78. 13 ± 6. 85） % and（90. 07 ± 7. 84） % respectively after 4 days,with the difference between the two groups being statistically significant（P 0. 001）. After treatment for 6 days,the survival rates of hypoxia model were（39. 01 ± 5.54） %,（60. 08 ± 7. 13） %,（80. 37 ± 5. 31） % and（92. 03 ± 7. 62） % respectively,with the difference between the two groups being statistically significant（P 0. 001）. The results of cell clone formation showed that the survival rate of microglia clones increased with the increase of resveratrol concentration. The survival rates of clones were（26. 47 ± 5. 66） %,（47. 92 ± 5. 07） %,（66. 09 ± 2. 93） % and（80. 07 ± 1. 49） % respectively after treatment for 7 days,with the difference between the two groups being statistically significant（P 0. 001）. Conclusions：Resveratrol could improve the survival of brain microglia under hypoxia.