甜菜MADS-box家族基因的全基因组鉴定及进化分析

Genome-wide Identification and Phylogenetic Analysis of MADS-box Family Gene in Beta vulgaris

为了进一步了解MADS-box家族基因的功能,利用生物信息学的手段,首次对甜菜MADS-box基因进行了全基因组的鉴定,并对其染色体定位、系统发生关系、基因结构、保守元件、表达模式以及蛋白功能联系进行预测和分析。结果表明,甜菜MADS-box基因共34个成员,其中,typeⅠ成员7个和typeⅡ成员27个,typeⅠ进一步分为Mα(3)、Mβ(1)、Mγ(3)3个组;typeⅡ进一步分为MIKCC(22)和MIKC*(5)2个组,MIKCC组可进一步分为AG(2)、AGL12(2)、AP3-PI(4)、Bs(2)、SOC1(1)、SVP(1)、SEP(3)、AGL17(5)、AP1-FUL(1)和FLC(1)10个亚组。MADS-box家族基因在染色体上呈不均匀分布,同一染色体上的基因簇状分布,其中,第6号染色体上分布最多,在第7号染色体上没有分布。甜菜MADS-box家族基因虽然基因结构差别较大,但蛋白序列相对保守。基因表达谱显示,大部分MADS-box基因优势表达于分生组织,部分MADS-box基因在种子、直根、幼叶等组织亦有较高表达。部分MADS-box基因响应盐、热胁迫轻微上调,可能参与甜菜逆境生理调控。

To further understand the function of MADS-box family gene,the genome-wide of beet MADS-box genes were first identified by bioinformatics,and chromosomal localization,phylogenetic relationship,gene structure,conserved domain,expression pattern and proteins function connections were predicted and analyzed. The results showed that there were 34 MADS-box genes in beet,among which 7 members belong to typeⅠand 27 members belong to typeⅡ. TypeⅠwas further divided into three class： Mα（ 3）,Mβ（ 1） and Mγ（ 3）,typeⅡwas further divided into two class： MIKCC（ 22） and MIKC＊（ 5）,and MIKCCclass were further divided into ten subclass： AG（ 2）,AGL12（ 2）,AP3-PI（ 4）,Bs（ 2）,SOC1（ 1）,SVP（ 1）,SEP（ 3）,AGL17（ 5）,AP1-FUL（ 1） and FLC（ 1）. The MADS-box genes showed uneven distribution on the chromosome,and genes cluster distribution on one chromosome. The most distributed was on chromosome 6,none on chromosome 7. The structure of the MADS-box genes was different,but the protein sequences were conserved relatively.The expression profile showed that most MADS-box genes were expressed highly in meristem,and some MADS-box genes were expressed advantageously in seeds,roots,young leaves,and so on. Some MADS-box genes are slightly up-regulated in response to salt or heat stress and may be involved in the physiological regulation under stress.