基于荧光差异双向电泳的雷帕霉素损伤人冠状动脉内皮细胞的蛋白质组学研究

Proteomics study of rapamycin-injured human coronary artery endothelial cells based on two dimension fluorescence differential gel electrophoresis

目的利用蛋白质组学的方法系统探索洗脱支架药物雷帕霉素损伤人冠状动脉内皮细胞的蛋白质谱的变化。方法雷帕霉素处理人冠状动脉内皮细胞HCAECs,进行荧光差异双向电泳(2D-DIGE)差异表达蛋白的研究分析,MALDI-ToF-ToF质谱对蛋白质进行鉴定。结果共发现差异蛋白斑点85个,其中上调49个,下调36个。MALDI-ToF-ToF质谱鉴定出其中的26种,包括内质网蛋白、线粒体蛋白、分子伴侣、结构蛋白和抗氧化蛋白等。结论通过蛋白质组学方法的研究,探索了雷帕霉素损伤人冠状动脉内皮细胞的一些特定蛋白质变化。

Objective To explore the molecular mechanisms of the effect of eluting stent drug rapamycin for injuring human coronary artery endothelial cells （HCAECs） by using the proteomics method. Methods HCAECs were treated with rapamycin,and the differentially expressed proteins were analyzed by two dimension fluorescence differential gel electrophoresis（2D-DIGE）. The changed proteins were identified by MALDI-ToF-ToF. Results At least 85 differential protein spots were found,including 49 up- regulated and 36 down-regulated protein spots. Twenty-six proteins were identified by MALDI-ToF-ToF,including the endoplasmic reticulum protein, mitochondrial protein, molecular chaperones, ubiquitin system related protein, structural protein and oxidative stress related proteins,etc. Conclusion The changes of specific proteins of HCAECs injury induced by rapamycin are investigated by the proteomic method.