过表达C3G对高糖诱导H9C2心肌细胞增殖和凋亡的影响

Effect of C3G overexpression on proliferation and apoptosis of high glucose-induced H9C2 cardiomyocytes

目的探讨过表达C3G对高糖诱导H9C2心肌细胞增殖和凋亡的影响。方法分别用pCXN2-Flag(空质粒)、pCXN2-Flag-hC3G(人过表达C3G mRNA)质粒通过瞬时转染H9C2心肌细胞,并进行高糖干预。实验分为空白组、空载体组、过表达C3G组、空白+高糖组、空载体+高糖组、过表达C3G+高糖组,分别检测各组H9C2心肌细胞的C3G蛋白表达、增殖率及凋亡率。结果空白+高糖组、空载体+高糖组较空白组、空载体组细胞增殖率均明显降低,凋亡率均明显升高。过表达C3G组较空白组与空载体组,过表达C3G+高糖组较空白+高糖组与空载体+高糖组H9C2心肌细胞的C3G蛋白表达增加,增殖率升高,凋亡率明显降低。结论高糖抑制H9C2心肌细胞增殖、促进H9C2心肌细胞凋亡;过表达C3G可逆转高糖诱导H9C2心肌细胞增殖率的降低、凋亡率的升高。过表达C3G能促进高糖诱导H9C2心肌细胞的生存力。

Objective To observe the effects of Crk-SH3 domain guanine exchange factor （C3G） overexpression on the pro- liferation and apoptosis of high glucose-induced H9C2 cardiomyocytes. Methods H9C2 cardiomyocytes were transiently transfected with pCXN2-Flag（empty plasmid） and pCXN2-Flag-hC3G （human C3G mRNA） plasmids,then conducted high glucose （HG） in- tervention. The experiment was divided into the blank group,empty vector group,C3G overexpression group, blank ＋ HG group, empty vector ＋ HG group and C3G overexpression ＋HG group. The C3G protein expressions, apoptosis and proliferation rate were respectively detected in each H9C2 cardiomyocytes groups. Results The Proliferation rate in the blank＋ HG group and empty vector ＋ HG group were significantly decreased compared with the blank group and empty vector group, While the apoptosis rate was significantly increased. Compared with blank group and empty vector group,blank ＋ HG and empty vector ＋ HG group,the C3G protein expression and proliferation rate in the C3G overexpression group and C3G overexpression＋HG group were increased significantly, while the apoptosis rate was decreased significantly. Conclusion High glucose inhibits H9C2 myocardial cell proliferation and promots its apoptosis;furthermore, C3G overexpression can reversed the decrease of high glucose-induced H9C2 cardiomyocyte proliferation rate and apoptosis increase. C3G overexpression can promote the survivability of high glucose-induced H9C2 cardiomyocytes.