摘要
目的探讨银杏内脂B(Ginkgolide B,GB)对大鼠缺氧模型焦虑样行为的改善作用。方法 36只SPF级SD大鼠随机分为平原对照组、高原缺氧组、GB干预组,每组12只。依托高原人工实验舱平台,以平原常氧条件饲养大鼠为平原对照组,以高原人工实验舱内模拟6000m高原缺氧环境,高原缺氧组和GB干预组在舱内6d,制造高原缺氧模型,其中GB干预组在进舱前4d以GB灌胃给药,每天给药剂量为120mg/kg。采用旷场实验(open field test,OFT)和高架十字迷宫(elevated plus maze,EPM)实验来观察GB对缺氧导致的大鼠焦虑行为的影响。结果在OFT中,平原对照组大鼠进入中央区域时间、次数以及运动总路程显著高于高原缺氧组,在EPM试验中进入开臂时间和次数百分比也显著高于高原缺氧组,组间比较差异具有统计学意义(P<0.05)。在OFT和EPM试验中,GB干预组的各项检测指标较高原缺氧组有显著的升高,组间比较差异具有统计学意义(P<0.05)。结论预先给予GB可以有效缓解高原缺氧导致的大鼠焦虑样行为。
Objective To investigate the intervention effects of Ginkgolide B（GB）on anxiety behaviors of SD rats induced by acute hypoxia.Methods Thirty-six SPF grade SD rats were separated into there groups：control group,acute hypoxia group and GB group,each 12 rats.Based on the plateau artificial laboratory module platform,the rats were fed on plains normoxic condition as the plain control group.The plateau hypoxia model was established in plateau hypoxia group and GB intervention group by exposing to 6000 metres altitude for 6 days,and GB group was previously given GB（12 mg/kg·d）lasting 4 days by gastric perfusion.The open filed test（OFT）and elevated plus maze（EPM）were applied to evaluate anxiety behaviors of acute hypoxia rats.Results The time of entering the central region,frequency and total exercise distance of plain control group in OFT were significantly higher than those of plateau hypoxia group（P〈0.05）.The percentage of open-arms time and frequency in EPM test were also significantly higher than these of plateau hypoxia group（P〈0.05）.The various indexes of GB group in both OFT and EPM tests were obviously higher than these of acute hypoxia group（P〈0.05）.Conclusion Pre-given GB can effectively alleviate the high altitude anoxia-induced anxiety-like behaviors in rats.
出处
《华南国防医学杂志》
CAS
2018年第1期1-4,共4页
Military Medical Journal of South China
基金
国家自然科学基金面上项目(81371444)
