p14基因启动子甲基化在上皮性卵巢癌临床检测中的意义

Significance of Promoter Hypermethylation of p14 Gene in the Clinical Detection of Epithelial Ovarian Cancer

目的:探究p14基因启动子异常甲基化检测在上皮性卵巢癌发生发展中的临床意义。方法:选取2007年9月~2009年6月我院收治的45例上皮性卵巢癌患者、30例卵巢良性肿瘤患者及20例卵巢组织正常者作为研究对象,通过甲基化特异性聚合酶链式反应法检测所有研究对象的p14基因启动子区甲基化情况,采用荧光定量PCR(FQ-PCR)检测组织标本p14mRNA表达,采用免疫组织化学法检测p14蛋白表达,并比较各组患者之间的差异。结果:正常卵巢组织、良性肿瘤组织和上皮性卵巢癌组织未检测到p14基因启动子区甲基化。p14mRNA在正常卵巢组织中的水平为(0.57±0.41),在卵巢癌组织的水平为(0.83±0.07),在良性肿瘤组织中的水平为(0.62±0.39),p14mRNA在卵巢癌组织中的水平与正常组织和良性肿瘤组织中的比较,差异有统计学意义,P<0.05;p14mRNA在正常组织和良性肿瘤组织中的水平比较,差异无统计学意义,P>0.05;p14表达在上皮性卵巢癌、良性卵巢肿瘤和正常卵巢组织中的阳性率分别为37.8%、73.3%和85.0%,三组中p14表达的阳性率比较,差异有统计学意义,P<0.05。结论:p14低表达可能与卵巢癌的发生、发展有关,但蛋白的异常表达与甲基化无关。

Objective： To study the significance of promoter hypermethylation of p14 gene in the clinical detection of the course of epithelial ovarian cancer. Methods： From September 2007 to June 2009, selected 45 cases of epithelial ovarian cancer patients, 30 patients with ovarian benign tumor and 20 patients with normal ovarian in our hospital as the research objects. The p14 Gene promoter region methylation of all the research objects was detected by methylation specific polymerase chain reaction method; the expression of p14mRNA was detected by fluorescent quantitative PCR（FQ-PCR）; and the expression of p14 protein was detected by immunohistochemistry, compared the differences between the three groups. Results： No hypermethylation was detected in p14 Gene promoter region about the epithelial ovarian cancer primary tissues, ovarian benign tumor and normal ovarian tissues. The amount of p14mRNA in normal ovarian tissues was（0.57±0.41）; in ovarian cancer tissues, it was（0.83±0.07）; in ovarian benign tumor tissues, it was（0.62±0.39）; the differences of p14mRNA between ovarian cancer tissues and normal tissues and benign tumor tissues were statistically significant, P〈0.05; the difference of p14mRNA between normal tissues and benign tumor tissues was not statistically significant, P〈0.05. The positive rate of p14 expression in epithelial ovarian cancer, ovarian benign tumor and normal ovarian tissues were 37.8%, 73.3%and 85%;compared the positive rate of p14 expression in the three groups, the differences were statistically significant, P 0.05.Conclusion： The lower expression of p14 Gene may be associated with the occurrence and development of ovarian cancer, but the abnormal protein expression may be not related to the hypermethylation.