急性白血病患者骨髓单个核细胞中芳香烃受体和色氨酸二加氧酶mRNA的表达

Correlation between the expression of aryl hydrocarbon receptor mRNA and tryptophan dioxygenase mRNA in patients with acute leukemia

目的探讨芳香烃受体(AHR)mRNA和色氨酸二加氧酶(TDO)mRNA在急性白血病患者骨髓单个核细胞中的表达及其相关性。方法选择2013年8月至2014年8月河南省人民医院收治的初诊急性白血病患者65例为观察组,其中急性髓系白血病(AML)50例,急性淋巴细胞白血病(ALL)15例;另选择同期因贫血就诊排除血液系统恶性疾病的患者15例为对照组。应用实时荧光定量反转录聚合酶链式反应法检测各组患者骨髓单个核细胞中AHR mRNA和TDO mRNA的表达,并分析二者的相关性。结果与对照组比较,观察组AML、ALL患者骨髓单个核细胞中TDO mRNA、AHR mRNA的表达均显著升高(P<0.05)。AML与ALL患者骨髓单个细胞中TDO mRNA、AHR mRNA的表达比较差异无统计学意义(P>0.05)。AML、ALL患者骨髓单个核细胞中TDO mRNA与AHR mRNA的表达均呈显著正相关(r=0.801、0.922,P<0.05)。AML、ALL患者的白细胞计数、血红蛋白含量、血小板计数及乳酸脱氢酶水平与TDO mRNA、AHR mRNA的表达均无显著相关性(P>0.05)。结论 TDO和AHR在急性白血病骨髓单个核细胞中呈高表达,TDO-KYN-AHR通路可能促进了急性白血病的发生、发展。

Objective To investigate the correlation between the expression of aryl hydrocarbon receptor(AHR) mRNA and tryptophan dioxygenase(TDO) mRNA in bone marrow mononuclear cells of patients with acute leukemia. Methods Sixty-five patients with newly diagnosed acute leukemia in Henan Provincial People' s Hospital from August 2013 to August2014 were selected as observation group,and there were 50 patients with acute myeloid leukaemia( AML) and 15 patients with acute lymphoblastic leukaemia( ALL). Fifteen patients with anemia were selected as control group in the same period( excluding the malignant disease of blood system). The expression of AHR mRNA and TDO mRNA in bone marrow mononuclear cells of patients in the groups was detected by real time fluorescence quantitative reverse transcription polymerase chain reaction. The correlation between AHR mRNA and TDO mRNA was analyzed. Results The expression of TDO mRNA and AHR mRNA in bone marrow mononuclear cells of AML and ALL patients in the observation group was significantly higher than that in the control group( P < 0. 05). There was no significant difference in the expression of TDO mRNA and AHR mRNA in bone marrow mononuclear cells between AML and ALL patients( P > 0. 05). There was significantly positive correlation between the expression of TDO mRNA and AHR mRNA in bone marrow mononuclear cells of AML and ALL patients( r = 0. 801,0. 922;P < 0. 05). The levels of white blood cell,hemoglobin,platelet and lactate dehydrogenase were not related to the expression of TDO mRNA and AHR mRNA in AML and ALL patients( P > 0. 05). Conclusion The expression of TDO and AHR in bone marrow mononuclear cells of acute leukemia patients is high,and the TDO-KYN-AHR pathway promotes the development of acute leukemia.