内蒙古奇乾地区媒介蜱携带伯氏疏螺旋体的调查研究

Investigation on Borrelia burgdorferii in ticks collected in Qiqian region of Inner Mongolia

目的了解内蒙古奇乾地区媒介蜱携带伯氏疏螺旋体(Borrelia burgdorferii,B.b)的感染情况。方法用布旗法采集游离蜱,利用PCR和基因测序法对媒介蜱标本中B.b的感染进行检测和基因分型。结果采集媒介蜱共320只,其中全沟硬蜱293只(91.56%),森林革蜱22只(6.88%),嗜群血蜱5只(1.56%)。媒介蜱B.b阳性携带率为26.88%,可携带B.garinii、B.afzelii和B.miyamotoi 3种基因型。其中全沟硬蜱、森林革蜱和嗜群血蜱B.b阳性携带率分别为29.01%(85/293)、4.55%(1/22)、0(0/5)。结论内蒙古奇乾地区全沟硬蜱为优势蜱种,携带B.b基因型存在多样性,应引起公共卫生部门的高度重视。

Objective To study the prevalence of Borrelia burgdorferii in ticks among Qiqian region of Inner Mongolia. Methods Free ticks were collected with the dragging method. PCR method and gene sequencing were used to detect the infection and genotyping of Borrelia burgdorferii in tick samples. Results A total of 320 ticks were collected, including 293 Ixodes persulcatus（91.56%）, 22 Dermacentor silvarum（6.88%） and 5 Haemaphysalis conicinna（1.56%）. The positive rate of tick-borne Borrelia burgdorferii was 26.88%, and there were three genotypes such as B.garinii, B.afzelii and B.miyamotoi. The positive rate of Ixodes persulcatus, Dermacentor silvarum, Haemaphysalis conicinna was 29.01%（85/293）, 4.55%（1/22）, 0（0/5）, respectively. Conclusions Ixodes persulcatus is the dominant species of ticks in Qiqian and carries various genotypes of Borrelia burgdorferii, which should be highly valued local public health authorities.