血管钠肽抑制肺动脉平滑肌细胞增殖及调控钠尿肽B受体表达的研究

Study of vasonatrin peptide inhibited the proliferation of pulmonary artery smooth muscle cell and regulated expression of natriuretic peptide B receptor

目的 :探讨血管钠肽 (VNP)对肺动脉平滑肌细胞 (PASMC)增殖及钠尿肽 B受体 (NPR-B)基因表达的影响。方法 :体外培养大鼠 PASMC,分别用噻唑蓝 (MTT)比色法、总蛋白含量测定、放免分析及打点杂交的方法 ,观察了VNP作用下 PASMC的 MTT吸光值、总蛋白含量、细胞内 c GMP、c AMP浓度和 NPR-B的 m RNA水平。结果 :VNP可以显著抑制血清刺激的 PASMC增殖 ,升高细胞内 c GMP浓度及 NPR-B m RNA水平 ,但对细胞内 c AMP浓度无明显影响。结论 :VNP对 PASMC增殖及 NPR-B基因表达具有调控作用 ,并且可能通过 c

AIM: To investigate the effect and mechanism of vasonatrin peptide (VNP) on the proliferation of pulmonary artery smooth muscle cell (PASMC) and gene expression of natriuretic peptide B receptor (NPR B), METHODS: The optical density, protein content, intracellular concentration of cGMP, cAMP and mRNA level of NPR B were measured by means of MTT colorimetry, protein colorimetry, radioimmunological analysis and dot hybridization respectively. RESULTS: VNP significantly inhibited the proliferation of PASMC stimulated by serum, elevated the concentration of cGMP and mRNA level of NPR B, but had no significant effect on the level of cAMP. CONCLUSION: VNP may regulate the proliferation and expression of NPR B in a cGMP dependent manner.