红景天对高海拔缺氧人体白细胞介素-6及基质金属蛋白酶-9的影响研究

Effects of compound Rhodiola sachalinensis A Bor pretreatment on serum interleukin-6 and matrix metalloproteinase-9 at high altitude regions

目的 观察红景天对急性高海拔缺氧人体白细胞介素-6（interleukin-6,IL-6）及基质金属蛋白酶-9（matrix metalloproteinase-9,MMP-9）含量的影响,探讨红景天提高低氧耐受能力,预防急性高原病的作用机制.方法 按照压力情况（海平面压力或模拟4000 m高海拔低压低氧条件）、运动情况（安静状态或踏阶运动）、口服红景天情况（口服红景天7 d或14 d）分别对8名受试者进行干预,分组分别为：常压安静组、常压运动组、低压安静组、低压运动组、红景天7 d运动组、红景天14 d安静组、红景天14 d运动组.检测不同状态下人体血清IL-6和MMP-9含量.结果 与常压安静组[IL-6为（27.93±1.24）pg/ml、MMP-9为（270.08±10.90）ng/ml]相比,低压安静组人体血清IL-6[（28.96±1.38）pg/ml]和MMP-9[（281.50±12.31）ng/ml]水平明显增高;与常压运动组相比[IL-6为（27.74±1.06）pg/ml、MMP-9为（269.88±9.90）ng/ml],低压运动组人体血清IL-6[（29.56±1.26）pg/ml]和MMP-9[（285.25±11.67）ng/ml]水平明显增高;差异均有统计学意义（P〈0.05）.低压运动组与低压安静组比较人体血清IL-6和MMP-9水平增高,差异有统计学意义（P〈0.05）.与低压运动组比较,在低压舱模拟4000 m高海拔条件下服用红景天7 d和14 d,受试者血清IL-6和MMP-9水平均明显降低,差异有统计学意义（P〈0.05）;服用红景天7 d和14 d人体血清IL-6和MMP-9水平差异无统计学意义（P〉0.05）.结论 低压条件下,人体运动后IL-6和MMP-9水平明显增高.IL-6和MMP-9可能参与了红景天对抗高海拔低氧低压环境影响人体运动能力的分子机制.

Objective To observe the effects of compound rhodiola sachalinensis A Bor （cRSAB） pretreatment on the serum levels of Interleukin -6 （ IL-6） and matrix metalloproteinase-9 （ MMP-9） at high altitude regions, so as to explore the mechanism of cRSAB for the elevation of hypoxia tolerance and the prevention of acute high altitude sickness .Methods Interventions were made on the 8 healthy subjects in accordance with different pressures （sea level pressure or simulated high altitude of 4000 m）, exercise （rest state or stepping-stool exercise） and oral taking of cRSAB （7 days or 14 days）.The subjects were divided into 7 groups： the conventional pressure at rest group （or group A）, the conventional pressure exercise group （or group B）, the hypobaric at rest group （or group C）; the hypobaric exercise group （or group D）, the oral-taking of cRSAB for 14 days at rest group （or group E）, the oral-taking of cRSAB for 7 days exercise group （or group F）, the oral-taking of cRSAB for 14 days exercise group （or group G）.Then, serum concentrations of IL-6 and MMP-9 at different conditions were detected by ELISA .Results As compared with those of group A[IL-6（27.93 ±1.24）pg/ml, MMP-9（270.08 ±10.90）ng/ml], the serum concentrations of IL -6 and MMP-9 in the subjects of group C [（28.96 ±1.38） pg/ml], MMP-9 [（281.50 ±12.31） ng/ml] were elevated significantly.When compared with those of group B [ IL-6 （27.74 ±1.06） pg/ml, MMP-9 （269.88 ± 9.90）ng/ml], the serum concentrations of IL -6 and MMP-9 in the group D subjects [（29.56 ±1.26）pg/ml], MMP-9 [（285.25 ±11.67） ng/ml] increased obviously.Statistical significance could be noticed , when comparisons were made between them （ P 〈0.05）.The serum concentrations of IL -6 and MMP-9 in the subjects of group C increased, as compared with those in the subjects of group D , with statistical significance （P 〈0.05）.At the simulated high altitude of 4000 m, the serum concentrations of IL-6 and MMP-9 in the subjects of group F and group G all decreased considerably , when compared with those in subjects of group D , also with statistical significance （P 〈0.05）.There was no statistical significance in serum concentrations of IL -6 and MMP-9, when comparisons were made between the subjects of group F and group G （ P 〉0.05）. Conclusions At hypobaric pressure, the serum concentrations of IL-6 and MMP-9 in the subjects with exercise increased obviously.IL-6 and MMP-9 might be associated with the mechanism of cRSAB in the protective effect of low pressures on human motor function .