容量超负荷心肌肥大时局部心肌AngⅡ,bFGF的变化及ATI受体拮抗剂对其影响的实验研究

Effect of myocardial bFGF,AngⅡ and ATI receptor antagonist on myocardial hypertrophy in the early stage of acute volum overload

目的 :观察急性容量超负荷心肌肥大 (MH)早期局部心肌碱性成纤维细胞生长因子 (b FGF)及血管紧张素 (Ang )的作用以及 Ang 1型受体 (AT1)拮抗剂 (Losartan,Los)的逆转 MH作用。方法 :选择 3 6只雄性 SD大鼠随机分为 3组 :腹腔动静脉造瘘容量超负荷组 (AVO组 )、容量超负荷治疗组 (L OS组 )及对照组 (CON组 ) ,每组 12只 ,对比检测术后第 10天和第 2 0天的收缩压 (SBP)、左室重量指数 (LVMI)、心肌羟脯氨酸 (HY)、b FGF,Ang 及血浆 Ang 含量。结果 :1ACF组 SBP和 L VMI第 10天和第 2 0天持续升高 (P<0 .0 5 ,vs CON组 ) ,给药后二者水平仍显著高于对照组 ,但较 ACF组明显下调 ;2 ACF组心肌 b FGF和 Ang 第 10和第 2 0天明显上升 (P<0 .0 5 ,vs CON组 ) ,AT1受体被阻断后第 10天心肌 b FGF显著升高 (P<0 .0 5 ,vs ACF组 ) ,第 2 0天无明显变化 (P>0 .0 5 ,vs ACF组 ) ,而心肌和血浆 Ang 较 ACF组明显增多 (P<0 .0 5 ) ;3心肌 b FGF,Ang 及 L VMI之间均呈显著正相关。结论 :心肌 b FGF可能和 Ang 共同参与急性容量超负荷 MH早期发生 ,其产生可能与心肌 Ang 有关 ,AT1受体拮抗剂早期具显著逆转

AIM:To determine the effects of myocardial basic fibroblast growth factor(bFGF),angiotensin Ⅱ(AngⅡ) and AngⅡ receptor 1(AT1) antagonist Losartan(LOS) on myocardial hypertrophy(MH) in the early stage of acute volume overload(AVO). METHODS:Abdominal aorta inferior vena cava fistula was performed in the rats of group AVO( n =12) and group LOS( n =12,Losartan was given once daily). All the indexes were measured at the 10th and 20th day and were compared with group Control(CON, n =12). RESULTS:①The levels of systolic blood pressure and left ventricle mass index(LVMI) were elevated evidently in group AVO and group LOS compared with group CON( P <0.05),while the levels in group LOS were lower significantly than in group AVO;② myocardial bFGF and AngⅡ increased obviously in group AVO ( P <0 05, vs group CON). Compared with group AVO,myocardial bFGF increased apparently at the 10th day( P <0.05),but did not change at the 20th day in group LOS( P >0.05),however,myocardial and plasma AngⅡ of group LOS were all elevated distinctly( P <0.05, vs group AVO);③There was obviously positive correlation among myocardial bFGF,AngⅡ and LVMI. CONCLUSION:Myocardial bFGF and AngⅡ may play an important role in early course of MH,and AT1 receptor antagonist (Losartan) may reverse MH.