基于表面增强拉曼光谱技术检测阿斯巴甜

Detection of Aspartamebased on the Surface-Enhanced Raman Spectroscopy

利用表面增强拉曼光谱检测水溶液和果汁中的阿斯巴甜(APM)。首先制备了银溶胶活性基底,并对相关特性进行了表征。其次对APM的拉曼特征峰进行了归属。然后通过对比性的实验研究确定了最佳促凝剂种类。最后对水溶液中的APM进行了表面增强拉曼光谱检测,获得了检测限,并利用逻辑回归分析方法实现了定量分析和回收率的测定。另外,当果汁中APM浓度低至1000mg/L时,仍能够清晰分辨出其特征峰,接近国家检测标准0.6g/kg。

In this paper,the detection of aspartame(APM)in aqueous solution and in fruit juice using surface-enhanced Raman spectroscopy(SERS)has been presented.The silver colloid(Ag col)active substrates were prepared,and the related properties were characterized firstly.Secondly,the Raman characteristic peaks of APM were classified,and then the optimum coagulant type was determined by comparative experimental study.Finally,the APM in aqueous solution were detected by SERS.The quantitative analysis was implemented by using logic regression analysis method.The limit of detection and recovery were determined at the same time.In addition,when the concentration of APM in juice is low to 1000 mg/L,it is still able to distinguish the characteristic peak,in close proximity to the national standard 0.6 g/kg.