摘要
目的探究非编码microRNA-195在脑膜瘤中的表达及其功能。方法采用原位杂交检测miR-195在脑膜瘤组织中的表达水平及其与临床分级的相关性;在脑膜瘤细胞株IOMM-Lee中过表达miR-195,通过CCK8、流式细胞术等检测miR-195对脑膜瘤细胞生物学功能的影响。结果 miR-195在脑膜瘤中表达,其表达水平与肿瘤级别呈负相关。在脑膜瘤细胞中过表达miR-195可显著抑制肿瘤细胞的细胞活力,将细胞周期阻滞于G1期;荧光素梅报告基因结果显示miR-195可以直接抑制c-myb的转录水平,在脑膜瘤细胞中过表达miR-195能够显著抑制c-myb mRNA水平和蛋白水平的表达。结论 miR-195在脑膜瘤中异常表达,与肿瘤临床分级相关,能够通过靶向调节c-myb的表达抑制脑膜瘤细胞的细胞周期,抑制肿瘤细胞的生长。
Objective To investigate the expression and function of non-coding microRNA-195（ miR-195） in meningioma.Methods In situ hybridization was used to measure the expression of miR-195 in meningioma and its correlation with clinical classification was analyzed. After miR-195 was overexpressed in meningioma IOMM-Lee cell line,CCK8 and flow cytometry were used to investigate the influence of miR-195 on the biological function of meningioma cells.Results MiR-195 was expressed in meningioma,and its expression level was negatively correlated with tumor grade. Overexpression of miR-195 in meningioma cells significantly inhibited the viability of tumor cells and helped to achieve cell cycle arrest in G1 phase. The results of luciferase reporter gene assay showed that miR-195 directly inhibited the transcriptional level of c-myb expression,and overexpression of miR-195 in meningioma cells significantly inhibited the mRNA and protein expression of c-myb.Conclusions MiR-195 is abnormally expressed in meningioma,which is correlated with clinical tumor classification. MiR-195 inhibits the cell cycle of meningioma cells and the growth of tumor cells by targeted regulation of the expression of c-myb.
出处
《国际神经病学神经外科学杂志》
北大核心
2017年第6期638-641,共4页
Journal of International Neurology and Neurosurgery
基金
泸州市科技计划项目[2016-S-67(6/23)]
