摘要
癌症 biomarker microRNAs (miRNAs ) 的敏感察觉具有为癌症诊断和治疗的重要重要性。尽管如此，在存在 miRNA 生物鉴定的察觉敏感被 miRNA 的结构的特征严重地限制(包括小长度和高顺序相同) 因为大多数这些方法基于目标扩大。此处，我们经由 nanoprobe 的唯一的策略向低丰富的 miRNA 的敏感、特定的察觉汇报一条新奇途径提高溶解的荧光扩大，展示分子的烽火结构的一根俘获探针在被设计。借助于这策略， miRNA-21 与象 1.38 fM 一样低的察觉限制从 10 fM 在一个线性范围被检测到 100 下午。最新发达的生物传感器的高选择被对一个目标的好歧视与单个底的失配表明。而且，这试金被用于低于 10.1% 在 90.2%-108% 的范围和变化的系数与恢复增加进胎儿的牛的浆液样品的 miRNA-21 的察觉，显示它到在新窗户中的 RNA 免疫分析和早癌症 diagnosis.Open
Sensitive detection of cancer biomarker microRNAs （miRNAs） is of vital importance for cancer diagnosis and treatment. Nonetheless, the detection sensitivity in the existing miRNA bioassays is severely limited by the structural characteristics of miRNA （including small length and high sequence homology） because most of these methods are based on target amplification. Herein, we report a novel approach to sensitive and specific detection of low-abundance miRNA via a unique strategy of nanoprobe dissolution-enhanced fluorescence amplification, in which a capture probe featuring molecular beacon structure is designed. By means of this strategy, miRNA-21 was detected in a linear range from 10 fM to 100 pM with a detection limit as low as 1.38 fM. High selectivity of the newly developed biosensor was demonstrated by the good discrimination against a target with a single-base mismatch. Furthermore, this assay was used for the detection of miRNA-21 added into fetal bovine serum samples with the recovery in the range of 90.2%--108% and coefficients of variation below 10.1%, indicating its promising applications to RNA immunoassays and early cancer diagnosis.
