防风多糖治疗骨质疏松大鼠的效果及对血清IL-6、TNF-α、TGFβ1、NO、NOS水平的影响

Effect of Polysaccharide on Osteoporosis in Rats and Its Effects on Serum Levels of IL-6,TN-alpha,TGF beta 1,NO and NOS

目的：探讨防风多糖治疗骨质疏松大鼠的实验效果及作用机制。方法：选取60只SPF级雌性SD大鼠,采用随机数字表分为假手术组（等量生理盐水）、模型组（等量生理盐水）、阳性对照组[乙烯雌酚0.05 mg/（kg·d）]、防风多糖低剂量组[250 mg/kg浓度防风多糖供试液,20 m L/（kg·d）、防风多糖高剂量组500 mg/kg浓度防风多糖供试液,20 m L/（kg·d）]各12只。结果：干预70 d后,模型组股骨干重较假手术组显著降低（P〈0.05）,阳性对照组、低剂量组、高剂量组的股骨干重大于模型组（P〈0.05）,高剂量组的股骨干重大于低剂量组（P〈0.05）;阳性对照组、低剂量组、高剂量组的血清IL-6、TNF-α水平低于模型组（P〈0.05）,TGF-β1、NO、NOS高于模型组（P〈0.05）;高剂量组的血清IL-6、TNF-α水平低于低剂量组（P〈0.05）,TGF-β1、NO、NOS高于低剂量组（P〈0.05）;阳性对照组、低剂量组、高剂量组的血清Ca^（2＋）、Mg^（2＋）、ALP水平低于模型组（P〈0.05）,P^（2-）高于模型组（P〈0.05）;高剂量组的血清Ca^（2＋）、Mg^（2＋）、ALP低于低剂量组（P〈0.05）,P^（2-）高于低剂量组（P〈0.05）。结论：防风多糖治疗骨质疏松大鼠能有效降低血清中钙、镁离子及ALP浓度,调节细胞因子,缓解去势大鼠的骨高分解状态,达到治疗效果。

Objective： To study the effect and mechanism of the treatment of rats with osteoporosis. Methods： A total of60 SPF grade female SD rats were randomly divided into sham operation group（ saline）,model group（ saline）,positive control group[DES 0. 05 mg/（ kg·d） ],ledebouriella polysaccharide low dose group[250 mg/kg concentration of Fangfeng polysaccharide test solution,20 m L/（ kg·d） ],wind polysaccharide high dose group [500 mg/kg concentration of Fangfeng polysaccharide test solution,20 m L（ kg·d） ],12 in each. Results： After 70 days,the model group＇s femoral shaft weight was significantly decreasedthan those in the sham operation group（ P 0. 05）. The positive control group,low dose group and high dose group＇s femur dry weight was greater than that of the model group（ P 0. 05）. The high dose group＇s femur dry weight was greater than the low dose group（ P 0. 05）. The positive control group,low dose group and high dose group＇s serum IL-6 and TNF-α levels were lower than those of the model group（ P 0. 05）.TGF-β1,NO and NOS beta 1 were higher than those in the model group（ P 0. 05）. The high dose group＇s serum IL-6 and TNF-α levels were lower than those of the low dose group（ P 0. 05）. TGF-β1,NO and NOS beta 1 were higher than those of the low dose group（ P 0. 05）. The positive control group,low dose group and high dose group＇s serum Ca^（2＋）,Mg^（2＋） and ALP were lower than those of the model group（ P 0. 05）. P^（2-） was higher than that of the model group（ P 0. 05）. The serum Ca^（2＋）,Mg^（2＋） and ALP of high dose group was than lower than those of the model group（ P 0. 05） and P^（2-） was higher than that of the low dose group（ P 0. 05）. Conclusion： Treatment of osteoporosis in rats with osteoporosis can effectively reduce the concentration of serum calcium,magnesium ions and ALP,regulate cyto-kines,relieve the bone of rats with osteoporosis,and achieve the therapeutic effect.