人参皂苷对大鼠成骨细胞增殖及成骨细胞中PINP、β-CTX分泌的影响

Effects of Ginsenosides on Proliferation of Osteoblasts and Secretion of PINP and β-CTX in Osteoblasts of Rats

目的：探讨人参皂苷对大鼠成骨细胞增殖及成骨细胞中I型前胶原氨基端前肽（PINP）、β-胶原降解产物（β-CTX）分泌的影响。方法：将出生24 h的SD大鼠头盖骨成骨细胞行体外原代及传代培养,分别在培养液中加入不同浓度（0、1、5、10、20μmol/L）的人参皂苷传代培养21 d,行茜素红染色并于倒置相差显微镜中观察成骨细胞矿化结节,应用Western blotting法测定大鼠成骨组织中PINP、β-CTX表达水平。结果：人参皂苷可促进大鼠成骨细胞增殖及矿化结节形成,且呈时间及浓度依赖性。随着人参皂苷浓度增加及作用时间延长,大鼠成骨细胞中PINP、β-CTX表达水平显著升高（P〈0.05）。结论：人参皂苷可促进大鼠成骨细胞增殖及矿化结节形成,其可能作用机制与人参皂苷可诱导成骨细胞组织中PINP、β-CTX分泌有关。

Objective： To investigate the effects of ginsenoside on the proliferation of rat osteoblasts and the secretion of type I procollagen amino terminal propeptide（ PINP） and β-collagen degradation product（ β-CTX） in osteoblasts.Methods： Sprague-Dawley（ SD） osteoblasts were cultured in vitro and subcultured in vitro for 24 h. Cultured cells were treated with different concentrations（ 0,1,5,10,20 μmol/L） for 21 days. The mineralized nodules of osteoblasts were observed under inverted phase contrast microscope. Alkaline phosphatase（ PINP） and β-CTX（ 10 μmol/L） were detected by Western blotting. Expression. Results： Ginsenoside could promote osteoblast proliferation and mineralized nodules formation in a dose-dependent and time-dependent manner. With the increase of ginsenoside concentration and the prolongation of the time,the expressions of PINP and β-CTX in osteoblasts of rats were significantly increased（ P 0. 05）. Conclusion： Ginsenoside can promote osteoblast proliferation and mineralized nodules formation in rats. The mechanism may be related to the secretion of PINP and β-CTX in ginsenosides-induced osteoblasts.