基于转录组测序的紫背天葵花青素相关基因分析

The Gynura bicolor Transcriptome as A Source for Anthocyanidin Gene Sequence Analysis

为获取正常生长状态下紫背天葵花青素合成相关基因,本研究以全株紫背天葵为试验材料,采用高通量测序技术(Illumina Hi Seq 2500)对紫背天葵全转录功能基因组测序后组装,再通过GO、Swiss-Prot和NR三大数据库进行花青素相关功能基因筛选,共获得29个花青素相关Unigene,包括17个花青素相关糖基转移酶、3个花青素酰基转移酶、6个花青素相关还原酶以及3个无色花色素双加氧酶(合酶)。聚类分析研究表明,涉及到花青素合成相关的四类酶成员的29个Unigene中,花青素酰基转移酶家族内部之间亲缘关系最近,无色花色素双加氧酶次之,花青素相关还原酶亲缘关系稍远,而花青素相关糖基转移酶家族成员亲缘关系最远。本研究获取的29个紫背天葵花青素相关Unigene信息,为进一步研究紫背天葵花青素生物合成过程及基因克隆等奠定了理论基础。

In order to explore Gynura bicolor correlated genes in biosynthesis pathway of anthocynidin under the normal conditions,this study selected all part of Gynura bicolor as experimental materials,the transcriptome of Gynura bicolor was sequenced by Illumina Hi Seq 2500 platform. 29 Unigenes related to anthocyanidin were obtainded by 3 databases including GO, Swiss-Prot and NR, which belonged to 4 kinds of genes involved 17 anthocyanidin relevant glucosyltransferases,3 anthocyanidin relevant acyltransferases, 6 anthocyanidin relevant reductases and 3 leucoanthocyanidin dioxygenases. The clustering analysis from 29 anthocyanidin Unigenes containing 4 kins of genes showed that anthocyanin acyltransferases had the closest relationship, followed by leucoanthocyanidin dioxygenase anthocyanin acyltransferases, then anthocyanin relevant reductases,and the genetic relation among anthocyanidin relevant glucosyltransferases is the farthest. The 29 unigenes related to anthocyanins biosynthesis are helpful for further research to anthocyanin biosynthesis process and gene cloning from Gynura bicolor.