BRCA1与Rad51的相互作用研究进展

The Research Progress of the Interaction of BRCA1 with Rad51

BRCA1基因是目前已知的一种十分重要的抑癌基因,是参与DNA损伤识别和修复的大型蛋白质复合物的一部分,它对细胞的正常生长、分化、DNA遗传物质的损伤修复以及保护基因组的完整性至关重要。BRCA1基因突变,会导致细胞生长机制紊乱,显著增加患癌率。Rad51(Hs Rad51)基因可以经由同源重组(HR)来修复双链断裂的生物遗传物质DNA。BRCA1与Rad51在生物体内相互作用,可有效抑制癌症发生。可采用荧光光谱、CD、NMR等方法来研究BRCA1与Rad51蛋白的相互作用。

BRCA1 is a very important tumor suppressor gene until now,which is a part of large protein complex to DNA damage identification and repair.It is essential for cells growth,differentiation,DNA genetic damage repair and protection of genome completeness.BRCA1 gene mutation will lead to the disorder of cells growth mechanism,and increase the cancer risk observably.The repair of DNA double strand breaks can be achieved by the Rad51(hsRad51) gene via homologous recombination(HR).BRCA1/Rad51 interactions in vivo can effectively inhibit the occurrence of cancer and the interaction between Rad51 and BRCA1 proteins can be identified by fluorescence spectroscopy,CD,NMR and other methods.