化学治疗联合靶向COX-2 siRNA对大鼠胃癌组织JAM-1、E-cadherin及β-catenin蛋白表达的影响

Effects of COX-2 siRNA combined with chemotherapeutics on expression of JAM-1,E-cadherin and β-catenin protein

目的探讨化学治疗联合靶向环氧合酶-2(COX-2)siRNA对大鼠胃癌组织连接附着分子1(JAM-1)、E钙黏素(E-cadherin)及β-连环素(β-catenin)蛋白表达的影响。方法 45只健康SD大鼠按照随机数字表法分为观察组、阴性对照组和对照组,每组各15只。观察组采用化学治疗联合COX-2siRNA转染的胃癌细胞接种干预,阴性对照组采用化学治疗联合阴性siRNA转染的胃癌细胞接种干预,对照组采用未经siRNA转染的胃癌细胞接种干预,比较3组大鼠胃癌组织的COX-2、JAM-1、E-cadherin及β-catenin蛋白表达水平。结果经COX-2siRNA转染的胃癌SGC7901细胞凋亡率显著高于阴性siRNA转染组(P=0.000)和未经转染的SGC7901组(P=0.000);观察组大鼠肿瘤体积抑制率[(75.8±5.4)%]显著高于阴性对照组[(11.9±4.7)%](P=0.000);观察组COX-2蛋白表达显著低于阴性对照组(P=0.000)和对照组(P=0.000);观察组大鼠肿瘤组织JAM-1、E-cadherin及β-catenin蛋白表达阳性率显著高于阴性对照组和对照组(P<0.05)。结论化学治疗联合靶向COX-2siRNA可有效抑制大鼠胃癌组织中的COX-2蛋白表达,从而抑制肿瘤组织侵袭转移相关黏附分子JAM-1、E-cadherin、β-catenin蛋白表达,提高治疗效果。

Objective This paper intends to explore the effects of COX-2 siRNA combined with chemotherapeutics on the expression of JAM-1,E-cadherin,andβ-catenin protein.Methods Forty five healthy SD rats were randomly divided into the observation group,negative control group,and control group.The observation group was treated with chemotherapy combined with COX-2 siRNA transfected gastric cancer cells,the negative control group was treated with chemotherapy with negative siRNA transfected gastric cancer cells and the control group was treated with gastric cancer cells without siRNA transfection.The changes of COX-2,JAM-1,E-cadherin andβ-catenin expression in the three groups were compared.Results The apoptotic rate of SGC7901 cells transfected with COX-2 siRNA was significantly higher than negative siRNA(P=0.000)and untransfected SGC7901(P=0.000).The tumor volume inhibition rate in the observation group[(75.8 ± 5.4)%]was significantly higher than RNA 提取试剂SV Total RNA Isolation System 购that in the control group [(11.9 ± 4.7)%(P=0.000).The expression of COX-2 protein in the observation group was significantly lower than that in the negative control group(P=0.000)and the control group(P=0.000).The positive rate of JAM-1,E-cadherin andβ-catenin protein expression in tumor tissue in the observation group was significantly higher than that of the negative control group and the control group(P<0.05).Conclusions COX2 siRNA combined with chemotherapeutics can inhibit the expression of COX-2 protein to inhibit the expression of JAM-1,E-cadherin andβ-catenin in gastric cancer tissue of rats,which may improve the therapeutic effect.