超高效液相色谱法测定小麦中脱氧雪腐镰刀菌烯醇的研究

The study of deoxynivalenol determining in wheat by UPLC

采用免疫亲和柱净化-超高效液相色谱法测定小麦中脱氧雪腐镰刀菌烯醇(DON)。结果表明:在100~1000 ng/mL,DON线性关系良好(R2=0.999 933),精密度RSD为1.3%;具有较高的回收率在93.0%~105.5%。本方法准确,快速、简便,能满足小麦样品中DON的检测。

The method of deoxynivalenol（DON）determining in wheat by UPLC was established.The results show that in the 100~1000 ng/mL,DON linear relationship is good（R2=0.999 933）,and the precision RSD is 1.3 %.It has a high recovery rate of 93.0% ~ 105.5%.This method is more accurate,quick and easy to meet the test of DON in wheat sample.