摘要
目的:探讨吉林人参低聚肽(GOP)对酒精诱导的大鼠急性酒精中毒的防治作用及其可能机制。方法:采用SPF级SD雄性大鼠,随机分为7组,每组10只,包括1个空白对照组、1个模型对照组、1个乳清蛋白对照组(0.250 0g/kg)和4个GOP剂量组(0.062 5、0.125 0、0.250 0、0.500 0g/kg)。连续灌胃30d后,采用体积分数50%的酒精以7g/kg BW剂量灌胃,观察大鼠翻正反射,测定大鼠血清ALT、AST、TC、TG、HDL-C和LDL-C的含量,及肝组织ADH与大鼠血清TNF-α、IL-6、IL-1β水平。结果:与模型对照组相比,人参低聚肽干预组大鼠翻正反射消失的比率、血清TNF-α、IL-6、IL-1β的水平明显降低(P<0.05),血清ALT、AST及肝组织ADH活性显著升高(P<0.05),但TC、TG、HDL-C和LDL-C含量与模型对照组相比无统计学差异(P>0.05)。结论:人参低聚肽可有效保护机体免受急性酒精中毒的伤害和防治急性肝损伤。
Objective To investigate the effects and mechanism of Panax ginseng oligopeptide（ GOP） on acute alcohol poisoning in rats. Method A total of 70 SD rats were randomly assigned to 7 groups,including a normal control group,a model group,a whey protein group（ 0. 250 0 g/kg） and 4 GOP intervention groups（ 0. 062 5,0. 125 0,0. 250 0,0. 500 0 g/kg）. 7 g/kg BW 50% v ethanol was given intragastricly after the 30 days of rat stomach-lavaging continuously. The behavioral studies of loss of righting reflex,alcohol dehydrogenase（ ADH） activity in liver,and serum markers were assayed. Result We found that the GOP treatment reduced the rate of loss of righting reflex and the levels of TNF-α,IL-6,IL-1β in serum（ P〈0. 05）,increased the levels of AST,ALT in serum and the contents of ADH in liver tissue（ P〈0. 05）. In addition,the TC,LDL-C,HDL-C,TG levels in serum had no significant changes in groups of GOP at each dose（ P〈0. 05）. Conclusion It suggested that GOP had significant protective effect on cute alcohol poisoning in rats and acute liver injury,and the underlying mechanism might be related to inhibit the inflammatory response.
出处
《中国食物与营养》
2018年第2期68-72,76,共6页
Food and Nutrition in China
基金
"十一五"国家科技支撑计划(项目编号:2006BAD27B087)
