嗪草酮与牛血清白蛋白的荧光作用研究

fluorescence effect research between Metribuzin and the bovine serum albumins

用荧光光谱法研究嗪草酮与牛血清白蛋白的结合反应,显示嗪草酮能强烈的猝灭牛血清白蛋白的荧光强度。当嗪草酮的浓度为2×10-5～3.2×10-4mol/L时,荧光猝灭程度与嗪草酮浓度呈线性关系,其线性方程为I=3.44167+0.8885C,相关系数r=0.9926,检出限为6.26×10-7mol/l。用该方法对嗪草酮进行了实际样品中其含量测定。并且测定了该反应的结合常数和结合位点数。给出了在实际生产中对农药嗪草酮使用的指导意见。

This artile discussed the respond of metribuine and the bovine serum albumins in fluorescence spectrographic methods,in the demonstrated metribuine can intense quench bovine serum albumins the fluorescence intensity.When the density of metribuine is 2 ×10-5-3.2×10-4mol/L,the fluorescence quenches the degree to assume the linear relations with the density of metribuine,its linear equation is I=3.44167+0.8885C,correlation coefficient r=0.9926,The detection limit is 6.26×10-7mol/L.We have carried on the actual determination with this method to metribuine.Measuring the equilibrium constant K and the number of binding sites n.