槟榔碱对Hacat细胞上皮模型通透性的影响及钙离子的调节作用研究

Effect of arecoline on the permeability of Hacat cell epithelia model and the regulation of calcium ion

目的研究槟榔碱对Hacat细胞上皮模型通透性的影响及Ca^(2+)对其的调节作用。方法建立Hacat细胞上皮体外模型;设置对照组(A组)、槟榔碱处理组(B组)及Ca^(2+)+槟榔碱处理组(C组),以荧光黄表观通透系数(P_(app))评价槟榔碱及Ca^(2+)对细胞模型通透性的影响。结果在B组,各小组荧光黄P_(app)均大于相应A组(P<0.05),且荧光黄P_(app)随槟榔碱浓度及干预时间增加而增大(P<0.05)。在C组,各小组荧光黄P_(app)分别比相同浓度和时间点的B组低(P<0.05),各小组荧光黄P_(app)均大于A组(P<0.05),且荧光黄P_(app)随槟榔碱浓度及干预时间增加而增大(P<0.05)。结论槟榔碱导致上皮屏障通透性增大,可能是口腔黏膜下纤维性变(oral submucous fibrosis,OSF)发病作用机制之一。Ca^(2+)预处理使槟榔碱增加上皮通透性的作用减弱。

Objective To study the effect of arecoline on the permeability of Hacat cell epithelia model in vitro and the adjustment of Ca^2＋ on it. Methods Establish the Hacat cell epithelia monolayer model in vitro by culturing Hacat cell. The models were divided into control group （group A）, arecoline treatment group （group B） and ＂calcium＋ arecoline＂ treatment group （group C ）. The values of Lucifer Yellow Papp were used to estimate the permeability changes of models after treatment with arecoline and calcium. Results In group B, the Lucifer Yellow Papp of each subgroup was higher than that of group A （P 〈 0.05） and the Lucifer Yellow Papp increased as the arecoline concentration and treatment time were increased （P 〈 0.05）. In group C, after pretreatment with calcium, the Lucifer Yellow Papp of each subgroup were lower than that of group B at corresponding concentration and time points （P 〈 0.05） ;while the Papp of each subgroup was higher than that of group A （P 〈 0.05）. Besides, the Lucifer Yellow Papp increased as the arecoline concentration and treating time were increased （P 〈 0.05）. Conclusion The increase of permeability of oral epithelia model by areeoline may play an important role in the pathogenesis and development of oral submucous fibrosis. The pretreatment with calcium, to some extent, can weaken the effect of arecoline on the permeability of Hacat cell monolayer model.