SMARCA5在子痫前期胎盘的表达

Expression of SMARCA5 in placenta of preeclampsia

目的比较正常妊娠和子痫前期胎盘上SMARCA5的表达,初步探讨SMARCA5与子痫前期的关系。方法选取2015年8月至2016年8月在海南省人民医院剖宫产分娩的正常晚孕妇女30例、轻度子痫前期孕妇21例及重度子痫前期孕妇18例。分别应用实时荧光定量-PCR(q RT-PCR)和免疫组化法检测正常妊娠和子痫前期胎盘SMARCA5 m RNA和蛋白的表达水平。结果 q RT-PCR结果显示,正常妊娠组、轻度子痫前期组和重度子痫前期组胎盘SMARCA5 m RNA表达水平分别为(1.05±0.21)、(0.60±0.06)和(0.38±0.01),轻度子痫前期组与正常妊娠组比较显著下降,并且重度子痫前期组与轻度子痫前期组比较也显著下降,差异均有统计学意义(P<0.01);免疫组化结果同样显示,SMARCA5蛋白在子痫前期胎盘表达下降。结论子痫前期胎盘组织中SMARCA5表达明显下降,提示SMARCA5可能参与子痫前期的发病。

Objective To compare the expression of SMARCA5 in placenta of preeclampsia （PE） and normalpregnancy, and to investigate the relationship between SMARCA5 expression and preeclampsia. Methods A total of30 cases of late normal pregnant women, 21 cases of pregnant women with mild preeclampsia and 18 cases of pregnantwomen with severe preeclampsia who underwent cesarean section delivery in Hainan General Hospital from August2015 to August 2016 were selected. The expression of normal pregnancy, SMARCA5 mRNA and protein in placenta ofpreeclampsia were detected by real-time quantitative PCR （qRT-PCR） and immunohistochemistry. Results The resultsof qRT-PCR showed that the expression levels of SMARCA5 mRNA in normal pregnancy group and mild preeclampsiagroup were respectively （1.05 ± 0.21）, （0.60 ± 0.06）, （0.38 ± 0.01）. The expression of SMARCA5 in mild preeclampsiagroup was significantly lower than that in normal group, and the expression of SMARCA5 in severe preeclampsia groupwere significantly decreased compared with mild preeclampsia group （P〈0.01）. The immunohistochemical results alsoshowed that the expression of SMARCA5 protein were decreased in preeclampsia placenta. Conclusion Expression ofSMARCA5 was significantly decreased in placenta of preeclampsia, which suggesting that SMARCA5 may be involvedin the pathogenesis of preeclampsia.